CAJ | 학술논문

用高效液相色谱法测定苹果果皮多种类胡萝卜素。以丙酮与石油醚提取色素，用Novapak C18(4.6 mm×150 mm)色谱柱，乙腈∶甲醇∶二氯甲烷(70∶20∶10)流动相，在450 nm下检测类胡萝卜素。结果表明，玉米黄质、叶黄素、番茄红素与β-胡萝卜素的回收率分别是94.27%～99.82%、84.56%～89.41%、87.53～93.54%与88.08%～93.24%。其中富士果仅检测到玉米黄质(5.9×10－3 μg/mm2)与β -胡萝卜素(5.4×10－3 μg/mm2)；蛇果(红星)只有玉米黄质(9.1×10－3 μg/mm2)。
용고효액상색보법측정평과과피다충류호라복소。이병동여석유미제취색소，용Novapak C18(4.6 mm×150 mm)색보주，을정∶갑순∶이록갑완(70∶20∶10)류동상，재450 nm하검측류호라복소。결과표명，옥미황질、협황소、번가홍소여β-호라복소적회수솔분별시94.27%～99.82%、84.56%～89.41%、87.53～93.54%여88.08%～93.24%。기중부사과부검측도옥미황질(5.9×10－3 μg/mm2)여β -호라복소(5.4×10－3 μg/mm2)；사과(홍성)지유옥미황질(9.1×10－3 μg/mm2)。
A reversed-phase high performance liquid chromatographic method was used to determine carotenoids in apple peel.Carotenoids were extracted from apple peel with acetone and petroleum ether.Using Novapak C18 column(4.6 mm×150 mm),acetonitrile∶methanol∶methylene chloride (70∶20∶10) as mobile phase,carotenoids were eluted and monitored at 450 nm.It was shown that recoveries of zeaxanthin,lutein,lycopene and β-carotene were 94.27%～99.82%,84.56%～89.41%，87.53%～93.54%，and 88.08%～93.24%.‘Fuji’ apple contained zeaxanthin (5.9×10－3 μg/mm2) and β-carotene (5.4×10－3 μg/mm2) and Red Delicious apple only zeaxanthin (9.1×10－3 μg/mm2).