CAJ | 학술논문

研究了不同金粉用量对小麦幼胚瞬间及稳定转化频率的影响，结果表明此实验系统的金粉用量以每枪500μg金粉为佳。对获得的T0及T1代植株的PCR及Southern分析证实了外源bar基因片段已经稳定整合至小麦基因组中；对T1代植株的L-PPT涂抹实验表明转化植株对该除草剂的抗性水平较低；分析了T0、T1及T2代植株的表型特征，结果表明T0代植株生长矮小、结实率低等表型方面的异常很可能只是一种由于生理及栽培原因所导致的暂时现象。
연구료불동금분용량대소맥유배순간급은정전화빈솔적영향，결과표명차실험계통적금분용량이매창500μg금분위가。대획득적T0급T1대식주적PCR급Southern분석증실료외원bar기인편단이경은정정합지소맥기인조중；대T1대식주적L-PPT도말실험표명전화식주대해제초제적항성수평교저；분석료T0、T1급T2대식주적표형특정，결과표명T0대식주생장왜소、결실솔저등표형방면적이상흔가능지시일충유우생리급재배원인소도치적잠시현상。
Effects of gold particle amount per bombardment (30, 100, 250,500 μg) on transient and stable transformation of immature wheat embryos, were studied. And 500 μg gold particle per bombardment is superior to others both in GUS transient expression and transgenic plant production (Fig. 2). PCR and Southern analyses of T0 and Tt plants confirmed the integration of foreign bar gene fragments into wheat genomes (Figs. 5～7). Tt plants smeared with L-PPT 200 mg/L showed a lowlevel of resistance to this herbicide (Plate Ⅰd). The comparison of phenotypic character of To, T1, T2 and their control plants showed that the phenotype abnormality of To plants is only a temporary phenomenon due to physiological and cultivated conditions, and the plants will recover from this abnormality partially or fully in the following progenes (Table 2 and Plate Ⅰ-e).