CAJ | 학술논문

目的　观察细胞周期素D1、Rb及p16在早期胃癌发生中的作用及相互关系。方法　采用免疫组化SP法对39例早期胃癌癌旁肠化组织及34例非癌胃粘膜肠化组织进行对比研究。结果　癌组织中33/39例(84.6%)显示细胞周期素D1过度表达，癌旁肠化组织也显示细胞周期素D1过度表达。12/39例p16表达阴性，3例呈弱阳性。26例Rb阳性的肿瘤中，15例显示p16不表达或低表达，而9例Rb阴性的肿瘤中则p16显示表达增高。结论　细胞周期素D1是早期胃癌发生过程中常见的分子异常，细胞周期素D1的激活及Rb的失活在早期胃癌中可共同存在；Rb失活与p16表达之间存在负相关关系；早期胃癌的发生可能与细胞周期素D1、p16及Rb负反馈调节环路的异常有关。
목적　관찰세포주기소D1、Rb급p16재조기위암발생중적작용급상호관계。 방법　채용면역조화SP법대39례조기위암암방장화조직급34례비암위점막장화조직진행대비연구。 결과　암조직중33/39례(84.6%)현시세포주기소D1과도표체，암방장화조직야현시세포주기소D1과도표체。12/39례p16표체음성，3례정약양성。26례Rb양성적종류중，15례현시p16불표체혹저표체，이9례Rb음성적종류중칙p16현시표체증고。 결론　세포주기소D1시조기위암발생과정중상견적분자이상，세포주기소D1적격활급Rb적실활재조기위암중가공동존재；Rb실활여p16표체지간존재부상관관계；조기위암적발생가능여세포주기소D1、p16급Rb부반궤조절배로적이상유관。
Objective To investigate the expression of cell division regulators p16, Rb and cyclin D1 in human early gasric carcinoma tissues and their role in tumor transformation and the correlation among p16, Rb and cyclin D1. Methods A comparative study was carried out by using immuno-histochemical techniques between the paracarcinomatous intestinal metaplasia of 39 cases of early gatric carcinoma and the non-carcinomatous gastric mucosal intestinal metaplasia tissues of 34 cases.Results Over expression of cyclin D1 was determined in 33/39 carcinomatous samples(84.6%) and also in para-carcinomatous intestinal metaplasia tissues. p16 was undetectable in 12 of 39 samples. Interestingly, 15 of 26 Rb positive cancers had no or low p16,while 9 Rb negative cancers showed high levels of p16.Conclusion The over expression of cyclin D1 may be a common molecular abnormality and an early molecular event in early gastric carcinoma. Cyclin D1 over expression and Rb inactivation can co-exist in early gastric carcinoma. However, there is a reciprocity between Rb inactivation and p16 expression in early gastric carcinoma. Thus, abnormality in the negative feedback regulatory pathway of cyclin D1,Rb and p16 may be related to the tumorigenesis in early gastric carcinoma.