CAJ | 학술논문

目的建立用于转基因小鼠种质保存的快速胚胎冷冻方法。方法用乙二醇作为抗冻保护剂，对携带HBV-C基因的转基因小鼠模型进行了玻璃化胚胎冷冻保存的研究。结果发现含体积分数30%乙二醇的冷冻液可达到最佳的冷冻效果，有81.54%的冷冻胚胎在体外可进一步发育，其中69.81%达到孵化阶段，比较不同发育阶段的胚胎的冷冻效果发现，致密桑椹胚解冻后的孵化率显著高于囊胚。不同的装管方法对胚胎的回改率也有影响，五段法的胚胎回收率显著高于二段法。将解冻后形态正常的胚胎82枚移植给8只假孕母鼠，5只怀孕，怀孕率为62.5%，产下28只小鼠，产仔率为54.9%。结论一步法玻璃化冷冻方法可用于转基因小鼠的种质保存。
목적 건립용우전기인소서충질보존적쾌속배태냉동방법。방법 용을이순작위항동보호제，대휴대HBV-C기인적전기인소서모형진행료파리화배태냉동보존적연구。결과 발현함체적분수30%을이순적냉동액가체도최가적냉동효과，유81.54%적냉동배태재체외가진일보발육，기중69.81%체도부화계단，비교불동발육계단적배태적냉동효과발현，치밀상심배해동후적부화솔현저고우낭배。불동적장관방법대배태적회개솔야유영향，오단법적배태회수솔현저고우이단법。장해동후형태정상적배태82매이식급8지가잉모서，5지부잉，부잉솔위62.5%，산하28지소서，산자솔위54.9%。결론 일보법파리화냉동방법가용우전기인소서적충질보존。
Objective To establish a simpl e method for germplasm storage oftransgenic mice by vitrification.Methods Ethylene glycol(EG) was used as a cryoprotectant for embryo cryopreservation of HBV-C transgenic mice by vitrification.Results The best results of cryopreservation were obtained after using vitrification solution containing 30% EG.The develop i ng percentage of thawed embryos were 81.54% and 69.81% of them developed to ex pa nded blastocysts.Comparing recovered compacted morulae with blastocyst in expan d ed blastocysts percentage.the former were significantly better than the latter( 7 8.95% vs 56.52%，P＜0.05).Different methods of installation of the 0.25-ml plasti c straw infulenced the recovery rate.When recovered embryos were transferred in to pseudo-pregnant recipients，54.9% embryos developed to live offspring.Conclusion This method may be of practical use for embryos preservation of transgenic mice.