CAJ | 학술논문

FSH、EGF是参与卵巢功能调节的重要因素。关于EGF对FSH促进的胚胎卵巢卵泡发育和类固醇激素发生的影响报道甚少。本实验以单个卵巢的总卵泡数、生长卵泡数和5个最大卵母细胞的平均直径为卵泡发育优劣的衡量指标，利用我们早期研究建立的无血清培养体系研究了EGF对FSH促进的小鼠胚胎卵巢卵泡发育和分泌雌二醇的作用。结果发现： EGF+FSH+雄烯二酮（A）处理组的胚胎卵巢总卵泡数在培养第7～9 d显著高于FSH+A处理组,而且生长卵泡数和卵巢内5个最大卵母细胞平均直径在培养后期也显著高于FSH+A处理组（P<0.05）； FSH+A处理组的胚胎卵巢在培养第7～9 d分泌大量雌二醇，而EGF和FSH联合使用时胚胎卵巢在培养早期雌二醇分泌水平显著低于FSH+A处理组（P<0.05）。结果提示EGF协同FSH促进小鼠胚胎卵巢卵泡体外生长发育；同时EGF抑制FSH诱导的雌二醇分泌，其促卵泡发育作用可能与雌二醇的合成不相关联。
FSH、EGF시삼여란소공능조절적중요인소。관우EGF대FSH촉진적배태란소란포발육화류고순격소발생적영향보도심소。본실험이단개란소적총란포수、생장란포수화5개최대란모세포적평균직경위란포발육우렬적형량지표，이용아문조기연구건립적무혈청배양체계연구료EGF대FSH촉진적소서배태란소란포발육화분비자이순적작용。결과발현： EGF+FSH+웅희이동（A）처리조적배태란소총란포수재배양제7～9 d현저고우FSH+A처리조,이차생장란포수화란소내5개최대란모세포평균직경재배양후기야현저고우FSH+A처리조（P<0.05）； FSH+A처리조적배태란소재배양제7～9 d분비대량자이순，이EGF화FSH연합사용시배태란소재배양조기자이순분비수평현저저우FSH+A처리조（P<0.05）。결과제시EGF협동FSH촉진소서배태란소란포체외생장발육；동시EGF억제FSH유도적자이순분비，기촉란포발육작용가능여자이순적합성불상관련。
Follicle-stimulating hormone（FSH） and Epidermal Growth Factor（EGF）are important regulatory proteins, which involve the regulation of ovarian function. Very few informations were reported on the regulation of EGF in follicle development and steroidogenesis of fetalovary. The present experiment was conducted to evaluate the regulation of EGF on the FSH-induced follicular development and estradiol secretion of mouse fetal ovary in a serum-free condition. The average number of all oocytes, the growing oocyte per-ovary and the average diameter of the five largest growing oocytes were appiled as the index of follicular development. The results revealed that:　The total number of oocyte in EGF+FSH+A group was significantly higher than that in FSH+A group between day 7 to 9 in the culture period （P<0.05）. The growing oocyte number and the average diameter of the five largest oocytes in EGF+FSH+A group were significantly prior to that in FSH+A group in thelate culture period （P<0.05）.　A higher level of estradiol in FSH+Agroup was detected from 7 to 9 culture days, while at the same periodonly low concentration of estradiol existed in EGF+FSH+A group. The results showed that EGF had a synergic role with FSH in follicular development of mouse fetal ovary, but which attenuates the production of estradiol induced by FSH in vitro. This suggested that the stimulatory effect of EGF on follicular development is independent of the estradiol production.