CAJ | 학술논문

构建了GST-PSP94融合蛋白表达质粒pGEX-PSP94，并使其在大肠杆菌中表达了GST-PSP94融合蛋白。该融合蛋白经亲合层析纯化后，免疫家兔获得了PSP94抗血清。经Wenstern blot分析，该PSP94抗血清可与重组PSP94蛋白发生特异性结合。用它对正常人和前列腺增生病人各10例、前列腺癌病人5例的外周血PSP94浓度进行了分析。结果显示，PSP94在这三种人外周血中的浓度无明显差异。提示PSP94不能作为前列腺癌的血清标志物。
구건료GST-PSP94융합단백표체질립pGEX-PSP94，병사기재대장간균중표체료GST-PSP94융합단백。해융합단백경친합층석순화후，면역가토획득료PSP94항혈청。경Wenstern blot분석，해PSP94항혈청가여중조PSP94단백발생특이성결합。용타대정상인화전렬선증생병인각10례、전렬선암병인5례적외주혈PSP94농도진행료분석。결과현시，PSP94재저삼충인외주혈중적농도무명현차이。제시PSP94불능작위전렬선암적혈청표지물。
Recombinant plasmid pGEX-PSP94 expressing fusion protein GST-PSP94 was constructed and expressed fusion protein GST-PSP94 in E.coli.Anti-PSP94 serum was obtained from immunized rabbits using GST-PSP94 protein after purified by affinity chromatography.Immunology analysis indicated that anti-PSP94 serum can specifically bind to PSP94 recombinant protein.Analysis of PSP94 concentration in human peripheral blood from ten normal persons,ten patients with benign prostate hyperplasia(BPH) and five cases with prostate carcinoma showed that no significant difference of PSP94 concentration is found in the serum from control and experimental groups.The results suggested that PSP94 can not be regarded as a serum marker of prostate carcinoma.