CAJ | 학술논문

目的　制备抗食管癌旁组织核基质蛋白的特异性单抗。方法　SDS-PAGE分析正常食管组织、食管癌旁组织及食管癌组织核基质蛋白间的差异。用食管癌旁组织核基质蛋白做免疫原免疫Balb/c小鼠，利用杂交瘤技术制备了5株能稳定分泌单克隆抗体的杂交瘤细胞株，并对9-1-2/D、9-1-7/D 2株细胞作了初步鉴定。结果　免疫组化分析发现9-1-2/D单抗与食管癌及正常食管组织均有反应，而9-1-7/D单抗只与食管癌组织反应，与正常食管组织无反应。Western Blotting分析显示： 9-1-2/D单抗与电泳图谱上正常食管组织核基质和食管癌旁组织核基质52 kD的蛋白反应，而与食管癌组织核基质55 kD的蛋白反应。9-1-7/D单抗只与电泳图谱上食管癌旁组织及癌组织核基质46 kD的蛋白反应，与正常食管组织核基质无反应。结论本实验为食管癌的早期诊断作了初步有意义的探讨。
목적　제비항식관암방조직핵기질단백적특이성단항。방법　SDS-PAGE분석정상식관조직、식관암방조직급 식관암조직핵기질단백간적차이。용식관암방조직핵기질단백주면역원면역Balb/c소서，이용잡교류기술제비료5주능은정 분비단극륭항체적잡교류세포주，병대9-1-2/D、9-1-7/D 2주세포작료초보감정。결과　면역조화분석발현9-1-2/D단항여식관 암급정상식관조직균유반응，이9-1-7/D단항지여식관암조직반응，여정상식관조직무반응。Western Blotting분석현시： 9-1-2/D단항여전영도보상정상식관조직핵기질화식관암방조직핵기질52 kD적단백반응，이여식관암조직핵기질55 kD적단 백반응。9-1-7/D단항지여전영도보상식관암방조직급암조직핵기질46 kD적단백반응，여정상식관조직핵기질무반응。결론 본실험위식관암적조기진단작료초보유의의적탐토。
Objective To prepare anti-nuclear matrix proteins of tissues surrounding esophagus cancer monoclonal anti- bodies. Methods Differences among nuclear matrix proteins (NMPS) of normal esophagus tissues, esophagus cancer tissues and tissues adjacent to cancer were identified by SDS-PAGE. Balb/c mouse were hyperimmunized with NMPS of tissues adja- cent to esophagus cancer. Five mouse lymphocyte hybridoma cell lines were established and preliminary identification of cell lines 9-1-2/D, 9-1-7/D were made. Results Immunohistochemical analysis showed monoclonal antibody 9-1-2/D reacted both with esophagus cancer and normal esophageal tissues; monoclonal antibody 9-1-7/D reacted only with nucleoli of esophagus cancer cells. Western Blotting analysis showed monoclonal antibody 9-1-2/D reacted with 52 kD NMPS of normal esophagus tissues and tissues adjacent to esophagus cancer and reacted with 55 kD NMP of esophagus cancer tissue. Monoclonal anti- body 9-1-7/D reacted only with 46 kD protein of esophagus cancer tissues and tissues adjacent to cancer. Conclusion Mono- clonal antibodies prepared by us might be a new method for early diagnosis of esophagus cancer.