CAJ | 학술논문

目的：探讨胃肠道恶性肿瘤及其切缘组织中c-myc基因mRNA和端粒酶活性表达与手术切缘的安全性关系。方法：用RT-PCR和TRAP方法检测12例胃癌和18例肠癌标本和切缘组织中c-myc基因mRNA和端粒酶活性的表达。结果：(1)胃肠道恶性肿瘤组织中c-myc基因mRNA和端粒酶活性有很高的表达率。(2)肿瘤的上下切缘组织中c-myc基因mRNA和端粒酶活性表达无明显差异。(3)在中心表达阳性的肿瘤中，距中心4 cm以上端粒酶均为阴性表达，而c-myc基因mRNA在距4 cm处仍有小部分呈阳性表达，5 cm处则呈阴性。结论：就c-myc基因mRNA表达和端粒酶活性表达而言，距离肿瘤5 cm可定为肿瘤的分子切缘。
목적：탐토위장도악성종류급기절연조직중c-myc기인mRNA화단립매활성표체여수술절연적안전성관계。방법：용RT-PCR화TRAP방법검측12례위암화18례장암표본화절연조직중c-myc기인mRNA화단립매활성적표체。결과：(1)위장도악성종류조직중c-myc기인mRNA화단립매활성유흔고적표체솔。(2)종류적상하절연조직중c-myc기인mRNA화단립매활성표체무명현차이。(3)재중심표체양성적종류중，거중심4 cm이상단립매균위음성표체，이c-myc기인mRNA재거4 cm처잉유소부분정양성표체，5 cm처칙정음성。결론：취c-myc기인mRNA표체화단립매활성표체이언，거리종류5 cm가정위종류적분자절연。
Objective: To detect the c-myc mRNA and telomerase activity(TA) expression in the tumor center and peripheral tissues of patients with gastrointestinal cancers, and to explore the security of tumor surgical margins. Methods: To detect the expression of c-myc mRNA and TA in tumor tissues ,the approximal and distal peripheral tissues, every lcm away from the tumor margin of 12 gastric and 18 colorectal carcinomas with RT-PCR and TRAP methods respectively.Results: (1)The expression rates of c-myc mRNA and TA in tumor tissues are high. (2)There are no remarkable differences of c-myc mRNA and TA expressions between higher or lower tumor surgical margins. (3)C-myc mRNA and TA can′t be detected in 5-cm away peripheral tissues. Conclusion: Considering C-myc mRNA and TA expression, 5 cm can be defined as molecular margin.