CAJ | 학술논문

用定量ELISA的方法检测背部携带分泌抗LDH-C4 IgG2b抗体杂交瘤小鼠的血清中IgG2b抗体浓度，结果表明：本法稳定，批间变异系数为7.04%～13.30%,批内变异系数为3.61%～10.20%；标准曲线相关性良好，相关系数从0.962?884～0.996?795；灵敏度较高，最低检出浓度为0.01?mg/L；特异性亦较好．
용정량ELISA적방법검측배부휴대분비항LDH-C4 IgG2b항체잡교류소서적혈청중IgG2b항체농도，결과표명：본법은정，비간변이계수위7.04%～13.30%,비내변이계수위3.61%～10.20%；표준곡선상관성량호，상관계수종0.962?884～0.996?795；령민도교고，최저검출농도위0.01?mg/L；특이성역교호．
The levels of IgG2b monoclonal antibodies against LD H-C4 in sera of mice bearing hybridoma backpack tumors secreting anti-LDH-C4-IgG2b were detected by quantitative ELISA． The accuracy between batches is 7.04%～l3.30 %, the intra-assay variation is 3.6l%～l0.20%． Standard curveof monoclonal lgG2b was well correlated (r=0.962　　884～0.996　　795)． The sensitivit y of the assay reach e dup to0.0l?mg/L． The present modified ELISA offers a reproducible, se nsitive, specific method in determination of antigen-specific IgG2b antibody in sera．