CAJ | 학술논문

[目的]观察不同浓度的大黄水煎剂对乌鳢离体小肠运动性能的影响,并探讨其药理作用机制.[方法]采用离体器官法,利用RM6240B生物信号采集与处理系统,记录不同浓度的大黄水煎剂对离体肠道作用前后的张力值.离体小肠平滑肌收缩曲线的描记;离体肠段下端固定在通氧气"L"形倒钩上,上端连接离体肌肉张力换能器,使肠段在恒温28 ℃今村液的麦氏浴槽内正常收缩.打开RM6240生物信号采集处理系统,以1 g砝码定标,待肠段收缩稳定后,先记录一段肠管在今村生理溶液恒温28 ℃时的收缩曲线5 min.在预试验的基础上依次将浓度为0.062、0 125、0.250、0.500 g/ml的大黄水煎剂分别取1 ml放入20 ml的今村液中混匀备用,然后分别记录各组加入不同稀释度大黄水煎剂后的收缩曲线5 min,观察肠管的收缩幅度(g)、收缩频率(次/min),并从收缩曲线的基线改变,观察小肠平滑肌张力变化,以收缩基线上升表示张力增强,收缩基线下降表示肌张力减弱.[结果]药液浓度为0.062和0.125 g/ml的大黄水煎剂对乌鳢离体肠道收缩表现出明显的增强作用;药液浓度为0.250和0.500 g/ml的大黄水煎剂对乌鳢离体肠道收缩表现出抑制作用,在用药浓度0.500 g/ml前后差异极显著.可见,大黄水煎剂对乌鳢离体小肠平滑肌自发收缩幅度在低剂量有明显的增强效应,表现为正性变力作用,并呈剂量依赖性;在高剂量时有明显的抑制效应,并呈剂量依赖性,但对收缩频率影响不明显.建议在实际养殖过程中饲料中添加大黄的量为0.062～0.125 mg/L,用于鱼类细菌慈宁宫疾病治疗,提高鱼体免疫机能.[结论]大黄水煎剂对乌鳢离体肠道平滑肌的收缩活动有明显的效应.
[목적]관찰불동농도적대황수전제대오례리체소장운동성능적영향,병탐토기약리작용궤제.[방법]채용리체기관법,이용RM6240B생물신호채집여처리계통,기록불동농도적대황수전제대리체장도작용전후적장력치.리체소장평활기수축곡선적묘기;리체장단하단고정재통양기"L"형도구상,상단련접리체기육장력환능기,사장단재항온28 ℃금촌액적맥씨욕조내정상수축.타개RM6240생물신호채집처리계통,이1 g겁마정표,대장단수축은정후,선기록일단장관재금촌생리용액항온28 ℃시적수축곡선5 min.재예시험적기출상의차장농도위0.062、0 125、0.250、0.500 g/ml적대황수전제분별취1 ml방입20 ml적금촌액중혼균비용,연후분별기록각조가입불동희석도대황수전제후적수축곡선5 min,관찰장관적수축폭도(g)、수축빈솔(차/min),병종수축곡선적기선개변,관찰소장평활기장력변화,이수축기선상승표시장력증강,수축기선하강표시기장력감약.[결과]약액농도위0.062화0.125 g/ml적대황수전제대오례리체장도수축표현출명현적증강작용;약액농도위0.250화0.500 g/ml적대황수전제대오례리체장도수축표현출억제작용,재용약농도0.500 g/ml전후차이겁현저.가견,대황수전제대오례리체소장평활기자발수축폭도재저제량유명현적증강효응,표현위정성변력작용,병정제량의뢰성;재고제량시유명현적억제효응,병정제량의뢰성,단대수축빈솔영향불명현.건의재실제양식과정중사료중첨가대황적량위0.062～0.125 mg/L,용우어류세균자저궁질병치료,제고어체면역궤능.[결론]대황수전제대오례리체장도평활기적수축활동유명현적효응.
[Objective] The aim of this study was to investigate effects of rhubarb decoction with different concentrations on the isolated intestinal movement performance of Ophicephalus argus,and to discuss its pharmacological mechanism.[Method] With RM6240C biological signal collection and processing system,tension values of the isolated intestine before and after the treatment by rhubarb decoction with different concentrations were recorded by the isolated organ method.[Result] Rhubarb decoction with concentration of 0.062 and 0.125 g/ml showed obvious enhancement on the isolated intestinal contraction of Ophicephalus argus,while rhubarb decoction with concentration of 0.250 and 0.500 g/ml showed inhibition on the isolated intestinal contraction of Ophicephalus argus.There was an extremely significant difference in rhubarb decoction with concentration of lower than 0.500 g/ml and more than 0.500 g/ml.With the increase of rhubarb concentration,the intestinal movement of Ophicephalus argus enhanced,but the contraction frequency did not change obviously.[Conclusion] Rhubarb decoction has remarkable effects on the contraction of smooth muscle in the isolated intestines of Ophicephalus argus.