第三军医大学学报
第三軍醫大學學報
제삼군의대학학보
ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE
2001年
1期
55-58
,共4页
杨光%周雅德%裴雪涛%李梁%冯凯%时维绢
楊光%週雅德%裴雪濤%李樑%馮凱%時維絹
양광%주아덕%배설도%리량%풍개%시유견
造血细胞%体外扩增%白介素11%基质细胞
造血細胞%體外擴增%白介素11%基質細胞
조혈세포%체외확증%백개소11%기질세포
目的 研究造血因子白介素11（IL-11）基因修饰的基质细胞对造血细胞体外扩增的影响。方法 采用逆转录病毒载体将IL-11基因转入基质细胞HFCL，用Northernblot检测基质细胞HFCLIL-11基因的表达，用流式细胞仪检测IL-11基因修饰的HFCL支持的脐血CD34+造血细胞体外扩增中表型为CD34+CD38-早期祖细胞和表型为CD34+CD41+巨核系定向祖细胞的比例。结果 基质细胞HFCL能够表达逆转录病毒介导的IL-11基因，并且在这种IL-11基因修饰的基质细胞支持下，脐血CD34+造血细胞经过7d扩增，扩增细胞中表型为CD34+CD38-的早期祖细胞和表型为CD34+CD41+巨核系祖细胞的比例分别为（1.62±0.23）%、（9.9±1.1）%，高于未转基因HFCL的（0.8±0.23）%、（6.5±1.8）%，而在相同条件下细胞因子支持的扩增细胞中则分别为（0.19±0.14）%、（6.0±1.1）%。结论 基质细胞能够表达经逆转录病毒载体介导的IL-11基因，而且经IL-11基因修饰的基质细胞能显著促进CD34+CD38-的早期祖细胞和CD34+CD41+巨核系祖细胞扩增。
目的 研究造血因子白介素11（IL-11）基因脩飾的基質細胞對造血細胞體外擴增的影響。方法 採用逆轉錄病毒載體將IL-11基因轉入基質細胞HFCL，用Northernblot檢測基質細胞HFCLIL-11基因的錶達，用流式細胞儀檢測IL-11基因脩飾的HFCL支持的臍血CD34+造血細胞體外擴增中錶型為CD34+CD38-早期祖細胞和錶型為CD34+CD41+巨覈繫定嚮祖細胞的比例。結果 基質細胞HFCL能夠錶達逆轉錄病毒介導的IL-11基因，併且在這種IL-11基因脩飾的基質細胞支持下，臍血CD34+造血細胞經過7d擴增，擴增細胞中錶型為CD34+CD38-的早期祖細胞和錶型為CD34+CD41+巨覈繫祖細胞的比例分彆為（1.62±0.23）%、（9.9±1.1）%，高于未轉基因HFCL的（0.8±0.23）%、（6.5±1.8）%，而在相同條件下細胞因子支持的擴增細胞中則分彆為（0.19±0.14）%、（6.0±1.1）%。結論 基質細胞能夠錶達經逆轉錄病毒載體介導的IL-11基因，而且經IL-11基因脩飾的基質細胞能顯著促進CD34+CD38-的早期祖細胞和CD34+CD41+巨覈繫祖細胞擴增。
목적 연구조혈인자백개소11（IL-11）기인수식적기질세포대조혈세포체외확증적영향。방법 채용역전록병독재체장IL-11기인전입기질세포HFCL，용Northernblot검측기질세포HFCLIL-11기인적표체，용류식세포의검측IL-11기인수식적HFCL지지적제혈CD34+조혈세포체외확증중표형위CD34+CD38-조기조세포화표형위CD34+CD41+거핵계정향조세포적비례。결과 기질세포HFCL능구표체역전록병독개도적IL-11기인，병차재저충IL-11기인수식적기질세포지지하，제혈CD34+조혈세포경과7d확증，확증세포중표형위CD34+CD38-적조기조세포화표형위CD34+CD41+거핵계조세포적비례분별위（1.62±0.23）%、（9.9±1.1）%，고우미전기인HFCL적（0.8±0.23）%、（6.5±1.8）%，이재상동조건하세포인자지지적확증세포중칙분별위（0.19±0.14）%、（6.0±1.1）%。결론 기질세포능구표체경역전록병독재체개도적IL-11기인，이차경IL-11기인수식적기질세포능현저촉진CD34+CD38-적조기조세포화CD34+CD41+거핵계조세포확증。
Objective To investigate the effect of IL-11 gene modifiedstromal cells on ex vivo expansion of hematopoietic cells. Methods After IL-11 gene was transferred into stromal cell line HFCL with retroviral vector pLXSN, the expression of IL-11 mRNA was detected with Northern blot in HFCL-IL-11 cells. The percentages of CD34+ CD38- early progenitor and CD34+ CD41+ megakaryocyte-committed progenitor were determined with flow cytometry after inoculation on stromal cell HFCL-IL-11. Results The HFCL cells expressed IL-11 mediated by retroviral vector. In the expansion of CD34+ cells from cord blood inoculated on HFCL-IL-11 cells for 7 d, the percentage of CD34+ CD38- early progenitors was (1.62±0.23)% and that of CD34+ CD41+ megakaryocyte-committed progenitors (9.9±1.1)%, both of which were higher than the expansion inoculated on untransferred HFCL cells [(0.80±0.23)%, (6.5±1.8)%, respectively] and higher than inoculated with cytokinases in the same conditions [(0.19±0.14)%, (6.0±1.1)%, respectively]. Conclusion Stromal cells can express the retroviral-mediated IL-11. These HFCL IL-11 cells can enhance the expansion of CD34+ CD38- early progenitor and CD34+ CD41+ megakaryocyte-committed progenitors.
