CAJ | 학술논문

以巴戟天无菌苗的茎尖、带节茎、无节茎为材料进行离体培养.结果表明:外植体表面消毒采用70%乙醇预处理30s,再用0.1%的升汞浸泡10～15min,效果较好.茎尖和带节茎培养,于含有6-苄基氨基嘌呤(6-benzylaminopurine.BA)的Murashige and Tucker(MT)培养基上,均能直接出芽,带节茎出芽率最高,为97.5%.适宜的BA浓度为1mg·L-1,BA浓度升高,出芽率下降.根的诱导,以MT加0.2～0.5 mg·L-1的萘乙酸(naphthlene acetic acid,NAA)培养基较好,生根率可达80.0%以上.
이파극천무균묘적경첨、대절경、무절경위재료진행리체배양.결과표명:외식체표면소독채용70%을순예처리30s,재용0.1%적승홍침포10～15min,효과교호.경첨화대절경배양,우함유6-변기안기표령(6-benzylaminopurine.BA)적Murashige and Tucker(MT)배양기상,균능직접출아,대절경출아솔최고,위97.5%.괄의적BA농도위1mg·L-1,BA농도승고,출아솔하강.근적유도,이MT가0.2～0.5 mg·L-1적내을산(naphthlene acetic acid,NAA)배양기교호,생근솔가체80.0%이상.