CAJ | 학술논문

本文采用同种大鼠颈部异位心脏移植模型研究受体胸腺内注射异基因骨髓细胞对移植心脏存活时间的影响及免疫学功能变化。实验分4组。对照组(Control组)、环磷酰胺组(CP组)、单纯胸腺内注射异基因骨髓细胞组(IT组)、胸腺内注射异基因骨髓细胞辅以短期环磷酰胺组(IT+CP组)。结果显示：移植心脏存活时间最长的是IT+CP组为68．3±13．4 d，CP组移植心脏存活时间为18．3±2．4 d，两组间移植心脏存活时间差异显著(P<0．01)，而二者分别与Control组比较差异显著(P<0．01，P<0．05)，Control组、IT组移植心脏存活时间较短，分别为8．5±1．4 d、7．8±1．2 d，受体接受实验处理因素1个月后免疫功能测定表明：各组大鼠脾细胞的淋巴细胞转化试验无明显差异(P>0．05)。IT+CP组大鼠脾细胞对供体脾细胞的单向混合淋巴细胞培养有明显抑制作用(P<0．01)，对供体靶细胞的细胞介导的细胞毒试验呈明显抑制作用(P<0．01)，说明受体对供体产生特异性免疫无反应性，即免疫耐受。
본문채용동충대서경부이위심장이식모형연구수체흉선내주사이기인골수세포대이식심장존활시간적영향급면역학공능변화。실험분4조。대조조(Control조)、배린선알조(CP조)、단순흉선내주사이기인골수세포조(IT조)、흉선내주사이기인골수세포보이단기배린선알조(IT+CP조)。결과현시：이식심장존활시간최장적시IT+CP조위68．3±13．4 d，CP조이식심장존활시간위18．3±2．4 d，량조간이식심장존활시간차이현저(P<0．01)，이이자분별여Control조비교차이현저(P<0．01，P<0．05)，Control조、IT조이식심장존활시간교단，분별위8．5±1．4 d、7．8±1．2 d，수체접수실험처리인소1개월후면역공능측정표명：각조대서비세포적림파세포전화시험무명현차이(P>0．05)。IT+CP조대서비세포대공체비세포적단향혼합림파세포배양유명현억제작용(P<0．01)，대공체파세포적세포개도적세포독시험정명현억제작용(P<0．01)，설명수체대공체산생특이성면역무반응성，즉면역내수。
In present study, heterotopic heart transplantation was performed by using the cuff technique, combined withintrathymic injection of donor bone marrow cells (ITBMC) to study the influence on allogeneic rat heart transplantation.The recipients (SD rat) were divided into four groups (n = 6 in each group), conrtol group、 group CP、 group IT、group IT + CP. The recipients received treatment at the time of heart transplantation. The result showed that the survivaltime of cardiac allograft in group IT + CP (68.3 + 13.4 days) was significantly longer than in control group (8.5 + 1.4days, P <0.01), group IT (7.8±1.2days, P <0.01) and group CP (18.3+2.4 days, P <0.01). The immunefunction of recipients was determined one month after intrathymic injection of allogeneic bone marrow cells. There was nodifference in the lymphocyte transformation test (LTT) in all groups ( P >0.05). There was a significant suppressiveeffect on mixed lymphecyet culture (MLC)(P <0.01) and lymphocyte mediated cytotoxicity (LMC) ( P <0.01) ingroup IT + CP comparing with control group. In conclusion, ITBMC combined with short course of cyclophosphamidecan induce donor- specific unresponsiveness in allogeneic rat cardiac transplantation.