CAJ | 학술논문

目的：研究驻极体对成纤维细胞表面电荷的影响及其细胞表面电荷与细胞生长、细胞凋亡的关系。方法：选用±300 V和±1 000 V驻极体分别作用成纤维细胞24、48和72 h，用流式细胞术和细胞电泳技术测定成纤维细胞的生长周期和细胞表面电荷。结果：(1)负极性驻极体作用成纤维细胞使细胞的电泳率和S期内的细胞数显著增加。(2)成纤维细胞表面负电荷和S期内细胞数的增加量与驻极体的表面电位成正比。(3)负极性驻极体能减少凋亡细胞的表面电荷量。(4)正极性驻极体作用成纤维细胞使G2和S期中的细胞数减少，同时减少了细胞表面的负电荷数量。结论：负极性驻极体具有促进成纤维细胞生长和增加细胞表面负电荷量的作用。正极性驻极体具有抑制成纤维细胞生长和降低细胞表面电荷密度的作用。凋亡细胞的表面电荷量较正常细胞要少。
목적：연구주겁체대성섬유세포표면전하적영향급기세포표면전하여세포생장、세포조망적관계。방법：선용±300 V화±1 000 V주겁체분별작용성섬유세포24、48화72 h，용류식세포술화세포전영기술측정성섬유세포적생장주기화세포표면전하。결과：(1)부겁성주겁체작용성섬유세포사세포적전영솔화S기내적세포수현저증가。(2)성섬유세포표면부전하화S기내세포수적증가량여주겁체적표면전위성정비。(3)부겁성주겁체능감소조망세포적표면전하량。(4)정겁성주겁체작용성섬유세포사G2화S기중적세포수감소，동시감소료세포표면적부전하수량。결론：부겁성주겁체구유촉진성섬유세포생장화증가세포표면부전하량적작용。정겁성주겁체구유억제성섬유세포생장화강저세포표면전하밀도적작용。조망세포적표면전하량교정상세포요소。
Objective: To study the influence of electret on surface charge of fibroblast cells (3T3 cells) and to probe the relationship between cell growth, apoptosis and cell surface charge. Methods: Electrets Teflon PTFE, ±300 V,±1 000 V were used to treat 3T3 cells for 24, 48 and 72 h. Then the influences of electrets on cell cycle and surface charge of 3T3 cells were studied by flow cytometry and electrophoresis, respectively. Results: (1) After 24 h action of negative electrets, electrophoretic mobility (or surface charge) and cell number in S phase of 3T3 cells were significantly increased compared with those in control group. (2) Effect of negative electrets enhancing cell growth and increasing cell surface charge was in proportional to the surface potential of electret. (3) Surface charge density of apoptotic cell was reduced by electret. (4) After 24 h action of positive electret, the cell number in S and G2 phase were decreased and cell surface charge was also reduced. Conclusion: Negative electret can improve cell growth and increase cell surface charge density. Positive electret can restrain cell growth and reduce cell surface charge density. Surface charge of apoptotic cell is less than that of normal cell.