中国天然药物
中國天然藥物
중국천연약물
CHINESE JOURNAL OF NATRUAL MEDICINES
2005年
6期
382-386
,共5页
黑鳗藤%黑鳗藤新苷A%溶血性%佐剂活性%增殖%抗体
黑鰻籐%黑鰻籐新苷A%溶血性%佐劑活性%增殖%抗體
흑만등%흑만등신감A%용혈성%좌제활성%증식%항체
Stephanotis mucronata%Stemucronatoside A%Haemolysis%Adjuvants%Proliferation%Antibody
目的:评价从黑鳗藤根中分离得到的C21甾体苷--黑鳗藤新苷A(stemucronatoside A,SA) 的溶血性及免疫佐剂作用.方法:以分光光度法测定黑鳗藤新苷A对血红细胞的溶血百分率;以卵清白蛋白(OVA) 100 μg,OVA 100 μg加氢氧化铝200 μg,OVA 100 μg加黑鳗藤新苷A 25 μg、50 μg和100 μg于第1 d和15 d分别免疫ICR小鼠,二免后14天,用MTT法检测刀豆蛋白A (Con A),脂多糖 (LPS)和OVA诱导脾淋巴细胞增殖反应,ELISA检测血清中的抗OVA抗体效价.结果:黑鳗藤新苷A显示轻微的溶血作用,其引起兔红细胞50%溶血的浓度(HD50)为331.39±26.16 μg/ml.黑鳗藤新苷A在剂量为50和100 μg时能显著增强Con A,LPS和OVA诱导的OVA受免小鼠脾淋巴细胞增殖反应(P<0.05 or P<0.01 or P<0.001);50 μg时能极显著提高OVA受免小鼠血清中OVA特异性抗体IgG,IgG1和 IgG2b的水平(P<0.01 or P<0.001).结论:黑鳗藤新苷A具有较低的溶血性和显著的免疫佐剂活性.
目的:評價從黑鰻籐根中分離得到的C21甾體苷--黑鰻籐新苷A(stemucronatoside A,SA) 的溶血性及免疫佐劑作用.方法:以分光光度法測定黑鰻籐新苷A對血紅細胞的溶血百分率;以卵清白蛋白(OVA) 100 μg,OVA 100 μg加氫氧化鋁200 μg,OVA 100 μg加黑鰻籐新苷A 25 μg、50 μg和100 μg于第1 d和15 d分彆免疫ICR小鼠,二免後14天,用MTT法檢測刀豆蛋白A (Con A),脂多糖 (LPS)和OVA誘導脾淋巴細胞增殖反應,ELISA檢測血清中的抗OVA抗體效價.結果:黑鰻籐新苷A顯示輕微的溶血作用,其引起兔紅細胞50%溶血的濃度(HD50)為331.39±26.16 μg/ml.黑鰻籐新苷A在劑量為50和100 μg時能顯著增彊Con A,LPS和OVA誘導的OVA受免小鼠脾淋巴細胞增殖反應(P<0.05 or P<0.01 or P<0.001);50 μg時能極顯著提高OVA受免小鼠血清中OVA特異性抗體IgG,IgG1和 IgG2b的水平(P<0.01 or P<0.001).結論:黑鰻籐新苷A具有較低的溶血性和顯著的免疫佐劑活性.
목적:평개종흑만등근중분리득도적C21치체감--흑만등신감A(stemucronatoside A,SA) 적용혈성급면역좌제작용.방법:이분광광도법측정흑만등신감A대혈홍세포적용혈백분솔;이란청백단백(OVA) 100 μg,OVA 100 μg가경양화려200 μg,OVA 100 μg가흑만등신감A 25 μg、50 μg화100 μg우제1 d화15 d분별면역ICR소서,이면후14천,용MTT법검측도두단백A (Con A),지다당 (LPS)화OVA유도비림파세포증식반응,ELISA검측혈청중적항OVA항체효개.결과:흑만등신감A현시경미적용혈작용,기인기토홍세포50%용혈적농도(HD50)위331.39±26.16 μg/ml.흑만등신감A재제량위50화100 μg시능현저증강Con A,LPS화OVA유도적OVA수면소서비림파세포증식반응(P<0.05 or P<0.01 or P<0.001);50 μg시능겁현저제고OVA수면소서혈청중OVA특이성항체IgG,IgG1화 IgG2b적수평(P<0.01 or P<0.001).결론:흑만등신감A구유교저적용혈성화현저적면역좌제활성.
AIM: To evaluate haemolytic activities and adjuvant effect of the C-21 steroidal glycoside-stemucronatoside A (SA) from the roots of Stephanotis mucronata. METHOD: The adjuvant potentials on the cellular and humoral immune responses to mice immunized with ovalbumin (OVA) were examined. ICR mice were immunized subcutaneously with OVA 100 μg alone, a mixture of OVA 100 μg and aluminoid 200 μg, or mixture of OVA 100 μg and SA 25, 50 or 100 μg on the first day and fifteenth day. Two weeks later (d 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific antibody in serum were investigated. RESULT: SA showed slight haemolytic activities with its concentration inducing 50% of the maximum haemolysis (HD50) being 331.39±26.16 μg/mL. SA significantly enhanced the Con A-, LPS-, and OVA -stimulated splenocyte proliferation in OVA-immunized mice at the doses of 50 and 100 μg (P<0.05 or P<0.01 or P<0.001). The OVA-specific IgG, IgG1 and IgG2b antibody levels in serum were significantly enhanced especially at the concentration of 50 μg compared with OVA control group (P<0.01 or P<0.001). CONCLUSION: SA possesses immunological adjuvant activities and low-haemolytic effect.
