CAJ | 학술논문

本研究测定了褐飞虱 Nilaparvata lugens、白背飞虱 Sogatella furcifera 和灰飞虱Laodelphax striatellus 的rDNA ITSl和ITS2的序列,以探讨这3种稻飞虱的分子鉴定方法.3种飞虱的ITSI和ITS2侧翼区(18S,5.8S和28S)序列相对稳定,但ITS1和ITS2序列在3种飞虱中变异较大.ITS1在所分析的438个位点中可变位点达294个,ITS2在分析的403个位点中可变位点为177个.根据3种飞虱rDNA的ITS1和ITS2序列设计了特异性引物,应用特异性引物对样品进行了PCR扩增,分析发现3种飞虱ITS1区的特异性引物扩增效果不理想.而ITS2区的特异性引物可以稳定地扩增出明显的目的DNA条带.因此,采用ITS2区的特异性引物可以对3种飞虱进行快速的分子鉴定.
본연구측정료갈비슬 Nilaparvata lugens、백배비슬 Sogatella furcifera 화회비슬Laodelphax striatellus 적rDNA ITSl화ITS2적서렬,이탐토저3충도비슬적분자감정방법.3충비슬적ITSI화ITS2측익구(18S,5.8S화28S)서렬상대은정,단ITS1화ITS2서렬재3충비슬중변이교대.ITS1재소분석적438개위점중가변위점체294개,ITS2재분석적403개위점중가변위점위177개.근거3충비슬rDNA적ITS1화ITS2서렬설계료특이성인물,응용특이성인물대양품진행료PCR확증,분석발현3충비슬ITS1구적특이성인물확증효과불이상.이ITS2구적특이성인물가이은정지확증출명현적목적DNA조대.인차,채용ITS2구적특이성인물가이대3충비슬진행쾌속적분자감정.
The complete sequences of rDNA ITS1 and ITS2 were determined for the brown planthopper Nilaparvata lugens, the white-backed planthopper Sogatella fiiTcifera and the small brown planthopper Laodelphax striatellus in order to explore the molecular identification method for them. The flanking regions of rDNA-ITSl and ITS2 of the three planthoppers showed only limited variation, but the sequences of rDNA-ITS1 and ITS2 differed significantly. There are 294 variable sites in the 438 analyzed sites for the ITS1 region, and 177 variable sites in the 403 analyzed sites for the ITS2 region. Species-specific primers of N. lugens, S. furcifera, and L striatellus were designed based on their rDNA-ITSl and ITS2 sequences. The results of PCR amplification of rDNA-ITSl in the three species indicated that the species-specific primers were not applicable. However, the species-specific primers based on the rDNA-ITS2 sequences proved to be useful diagnostic primers for the three planthoppers. It is so concluded that molecular identification of N. lugens, S. furcifera and L striatellus using the species-specific primers from the rDNA-ITS2 region is feasible.