湖北农业科学
湖北農業科學
호북농업과학
2010年
1期
182-185
,共4页
王学贵%叶娜%沈丽淘%杨继芝%龚国淑%张敏
王學貴%葉娜%瀋麗淘%楊繼芝%龔國淑%張敏
왕학귀%협나%침려도%양계지%공국숙%장민
菜青虫%乐果%农药残留%酶抑制-比色法
菜青蟲%樂果%農藥殘留%酶抑製-比色法
채청충%악과%농약잔류%매억제-비색법
Pieris rapae%dimethoate%pesticide residue%enzyme inhibition-colorimetry
运用酶抑制-比色法,以菜青虫乙酰胆碱酯酶作为试验酶源,测定了四川雅安地区的四季豆、生菜、凤尾等5种蔬菜中乐果的残留量.结果表明,生菜乙醇萃取液的农药残留量最大,达到4.209 3mg/kg,超过国家标准(1 mg/kg);凤尾农药残留量最低,其乙醇萃取液的农药残留量为0.043 8 mg/kg.此法对农药残留快速检测有一定的实践意义.
運用酶抑製-比色法,以菜青蟲乙酰膽堿酯酶作為試驗酶源,測定瞭四川雅安地區的四季豆、生菜、鳳尾等5種蔬菜中樂果的殘留量.結果錶明,生菜乙醇萃取液的農藥殘留量最大,達到4.209 3mg/kg,超過國傢標準(1 mg/kg);鳳尾農藥殘留量最低,其乙醇萃取液的農藥殘留量為0.043 8 mg/kg.此法對農藥殘留快速檢測有一定的實踐意義.
운용매억제-비색법,이채청충을선담감지매작위시험매원,측정료사천아안지구적사계두、생채、봉미등5충소채중악과적잔류량.결과표명,생채을순췌취액적농약잔류량최대,체도4.209 3mg/kg,초과국가표준(1 mg/kg);봉미농약잔류량최저,기을순췌취액적농약잔류량위0.043 8 mg/kg.차법대농약잔류쾌속검측유일정적실천의의.
The residue of dimethoate in 5 vegetables(Chinese cabbage,lettuce,Pteris,kidney beans,cabbage)from Ya'an was extracted with ethanol and detected by enzyme inhibition and colorimetry which used acetylcholinesterase from Pieris rapae larvae.The results indicated that the content of dimethoate in lettuce was the highest one,which exceeded the Chinese government standard of 1 mg/kg and reached 4.209 3 mg/kg.But the residue in Pteris as the minimum one was only 0.043 8 mg/kg.Some practical meanings were obtained for rapid detecting pesticide residues in this paper.