色谱
色譜
색보
CHINESE JOURNAL OF CHROMATOGRAPHY
2010年
1期
78-83
,共6页
白新伟%王延宝%赵怀鑫%孙志伟%夏莲%付艳艳%索有瑞%李玉林%尤进茂
白新偉%王延寶%趙懷鑫%孫誌偉%夏蓮%付豔豔%索有瑞%李玉林%尤進茂
백신위%왕연보%조부흠%손지위%하련%부염염%색유서%리옥림%우진무
非水毛细管电泳%柱前衍生化%9%10-菲醌%脂肪醛%饮料
非水毛細管電泳%柱前衍生化%9%10-菲醌%脂肪醛%飲料
비수모세관전영%주전연생화%9%10-비곤%지방철%음료
nonaqueous capillary electrophoresis%pre-column derivatization%9,10-phenanthrenequinone%aliphatic aldehydes%beverage
以9,10-菲醌作为柱前衍生试剂,采用非水毛细管电泳模式考察并优化了脂肪醛的分离条件.实验采用毛细管(总长58.5 cm,有效长度50 cm,内径50 μm),应用80 mmol/L NH4Ac、1.4 mol/L HAc缓冲体系,于20 ℃、5 kPa(50 mbar)下压力进样 8 s,在不加入其他添加剂的情况下,实现了7种脂肪醛的高效基线分离,检出限为0.37～0.87 μmol/L,线性范围为0.78～25 μmol/L.应用所建立的方法对实际样品进行了测定,结果令人满意.
以9,10-菲醌作為柱前衍生試劑,採用非水毛細管電泳模式攷察併優化瞭脂肪醛的分離條件.實驗採用毛細管(總長58.5 cm,有效長度50 cm,內徑50 μm),應用80 mmol/L NH4Ac、1.4 mol/L HAc緩遲體繫,于20 ℃、5 kPa(50 mbar)下壓力進樣 8 s,在不加入其他添加劑的情況下,實現瞭7種脂肪醛的高效基線分離,檢齣限為0.37～0.87 μmol/L,線性範圍為0.78～25 μmol/L.應用所建立的方法對實際樣品進行瞭測定,結果令人滿意.
이9,10-비곤작위주전연생시제,채용비수모세관전영모식고찰병우화료지방철적분리조건.실험채용모세관(총장58.5 cm,유효장도50 cm,내경50 μm),응용80 mmol/L NH4Ac、1.4 mol/L HAc완충체계,우20 ℃、5 kPa(50 mbar)하압력진양 8 s,재불가입기타첨가제적정황하,실현료7충지방철적고효기선분리,검출한위0.37～0.87 μmol/L,선성범위위0.78～25 μmol/L.응용소건립적방법대실제양품진행료측정,결과령인만의.
A simple and mild method for the separation of aliphatic aldehydes based on a condensation reaction with 9,10-phenanthrenequinone as labeling reagent with nonaqueous capillary electrophoresis has been developed. The detection was performed with a diode array detector (DAD). A 58.5 cm (50 cm effective length)×50 μm i. d. untreated fused-silica capillary was used. To optimize the conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The results indicated that the buffer concentration had a great impact on the separation, but the influences of temperature and added additives on the resolution were not obvious. The optimized conditions were as follows: 80 mmol/L ammonium acetate, 1.4 mol/L acetic acid, voltage of 28 kV, column temperature of 20 ℃ and DAD detection at 254 nm. The samples were introduced atmospherically with the injection at 5 kPa (50 mbar) for 8 s. The results indicate that seven aliphatic aldehyde derivatives and actual samples can be achieved baseline resolution under the proposed conditions.
