CAJ | 학술논문

为研究中华稻蝗种群遗传多样性和遗传结构,文章对我国7省市的7个中华稻蝗种群进行AFLP分析.选取7对引物扩增128个个体,共产生336条带,多态性条带292条,占86.90%.结果表明:中华稻蝗种群具有较高的遗传多样性水平,其中海南万宁种群的遗传多样性高于其他各种群.Mantel检验(r=0.27,P=0.89)表明中华稻蝗各种群遗传距离与地理距离间没有显著相关性.种群间具有明显的遗传分化现象.UPGMA(Unweighted pair group method average)聚类分析显示,7个中华稻蝗种群按地理距离分为3支:北方北京昌平、山西太原和山东济宁为一支;南方陕西汉中、湖南长沙和广西来宾为一支;海南万宁单独为一支.上述结果以及PCA(Principal component analysis)分析均表明由于地理隔离中华稻蝗种群显示出明显的南北分化和岛屿大陆种群遗传分化现象.
위연구중화도황충군유전다양성화유전결구,문장대아국7성시적7개중화도황충군진행AFLP분석.선취7대인물확증128개개체,공산생336조대,다태성조대292조,점86.90%.결과표명:중화도황충군구유교고적유전다양성수평,기중해남만저충군적유전다양성고우기타각충군.Mantel검험(r=0.27,P=0.89)표명중화도황각충군유전거리여지리거리간몰유현저상관성.충군간구유명현적유전분화현상.UPGMA(Unweighted pair group method average)취류분석현시,7개중화도황충군안지리거리분위3지:북방북경창평、산서태원화산동제저위일지;남방협서한중、호남장사화엄서래빈위일지;해남만저단독위일지.상술결과이급PCA(Principal component analysis)분석균표명유우지리격리중화도황충군현시출명현적남북분화화도서대륙충군유전분화현상.
In order to study the genetic diversity and genetic structure of populations,7 populations of Oxya chinensis from 7 provinces(or cities)of China were analyzed using AFLP technique.A total of 336 reproducible bands were amplifled with 7 primer combinations from 128 individuals.Two hundred and ninety-two bands(86.90%) were polymorphic.High genetic diversity was found among O.chinensis populations and Wanning population had higher genetic diversity than other populations.Mantel test(r=0.27,P=0.89)suggested that there was no significant association between genetic distance and geographic distance.Remarkable genetic differentiation was found among populations.Unweighted pair group method average(UPGMA)tree showed that the 7 O.chinensis populations were divided into 3 groups:Changping of Beijing,Taiyuan of Shanxi and Jining of Shandong populations in the north;Hanzhong of Shaanxi,Changsha of Hunan and Laibin of Guangxi populations in the south;and Wanning of Hainan population.Principal component analysis indicated significant genetic differentiation of the north and the south populations and island and continent populations existed in the 7 O.chinensis populations because of geographic isolation.