人参研究
人參研究
인삼연구
RENSHEN YANJIU
2011年
3期
5-7
,共3页
杨大鹏%李青%刘思杰%边疆%曹志强
楊大鵬%李青%劉思傑%邊疆%曹誌彊
양대붕%리청%류사걸%변강%조지강
人参%多糖%紫外分光光度法%苯酚-浓硫酸比色法
人參%多糖%紫外分光光度法%苯酚-濃硫痠比色法
인삼%다당%자외분광광도법%분분-농류산비색법
Conclusion： phenol-sulfuric acid colorimetric determination of ginseng polysaccharide%the method is simple%accurate%good stability
目的测定人参中人参多糖的含量,并建立人参多糖的含量测定方法。方法采用紫外分光光度法,苯酚-浓硫酸比色法于波长486nm处测定吸光度。结果人参多糖含量为13.31g/100g,标准曲线的线性范围为0.9991~0.9998,平均加样回收率为98.2%,RSD=2.23%。结论苯酚-浓硫酸比色法测定人参中多糖含量,方法简便、准确、稳定性好。
目的測定人參中人參多糖的含量,併建立人參多糖的含量測定方法。方法採用紫外分光光度法,苯酚-濃硫痠比色法于波長486nm處測定吸光度。結果人參多糖含量為13.31g/100g,標準麯線的線性範圍為0.9991~0.9998,平均加樣迴收率為98.2%,RSD=2.23%。結論苯酚-濃硫痠比色法測定人參中多糖含量,方法簡便、準確、穩定性好。
목적측정인삼중인삼다당적함량,병건립인삼다당적함량측정방법。방법채용자외분광광도법,분분-농류산비색법우파장486nm처측정흡광도。결과인삼다당함량위13.31g/100g,표준곡선적선성범위위0.9991~0.9998,평균가양회수솔위98.2%,RSD=2.23%。결론분분-농류산비색법측정인삼중다당함량,방법간편、준학、은정성호。
To determine the ginseng in the ginseng polysaccharide,ginseng polysaccharides and the establishment of the assay method.Methods：UV spectrophotometry,phenol–sulfuric acid at the wavelength of 486nm absorbance.The results of ginseng polysaccharide content13.31g/100g,the standard curve linear range 0.9991-0.9998,average recovery was 98.2%,RSD=2.23%.
