CAJ | 학술논문

应用高效液相色谱法（high performance liquid chromatography, HPLC）和SOS显色反应测定36株益生菌脱除4-硝基喹啉-i-氧化物（4-NQO）基因毒性的能力，同时用HPLC对它们的图谱进行比较分析。研究发现，基因毒性清除率高的菌株有1株植物乳杆菌、5株双歧杆菌、干酪乳杆菌代田株、3株芽孢杆菌、2株酵母菌、7株唾液乳杆菌和一株保加利亚乳杆菌。同属于一个菌属的各个菌株基因毒性清除率之间差异不显著。图谱分析发现，只有唾液乳杆菌才产生P1，即益生菌脱除4-NQO基因毒性具有菌株特异性。研究还发现益生菌转化4-NQO为4-HAQO的能力是决定益生菌脱除4-NQO基因毒性能力大小的关键。
응용고효액상색보법（high performance liquid chromatography, HPLC）화SOS현색반응측정36주익생균탈제4-초기규람-i-양화물（4-NQO）기인독성적능력，동시용HPLC대타문적도보진행비교분석。연구발현，기인독성청제솔고적균주유1주식물유간균、5주쌍기간균、간락유간균대전주、3주아포간균、2주효모균、7주타액유간균화일주보가리아유간균。동속우일개균속적각개균주기인독성청제솔지간차이불현저。도보분석발현，지유타액유간균재산생P1，즉익생균탈제4-NQO기인독성구유균주특이성。연구환발현익생균전화4-NQO위4-HAQO적능력시결정익생균탈제4-NQO기인독성능력대소적관건。
SOS chromotest and HPLC were used for determining the antigenotoxic activity of 36 probiotic strains against 4-NQO. Meanwhile, their ability to remove 4-NQO genotoxicity was comparatively analyzed by HPLC. One L. plantarum strain, 5 Bifidobacterium strains, L. casei strain Shirota, 3 Bacillus strains, 2 yeast strains, 7 Lsalivarius strains and I Lactobacillus bulgaricus strain were found to have a higher ability to remove 4-NQO genotoxicity among these strains. No significant difference in removal ability for 4-NQO genotoxicity was observed among strains from the same genus. Only L.salivarius strains could produce Px, suggesting the species-specific removal ability of probiotics for 4-NQO genotoxicity. Also, we found that the capability of converting 4-NQO into 4-HAQO decided the ability of probiotics to remove 4-NQO genotoxicity. This study can provide references and supports for further studies of the removal mechanism of 4-NQO genotoxicity by probiotics.