CAJ | 학술논문

目的探讨多次加热后对残存的肝癌HepG2细胞的谷胱苷肽-S-转移酶π(GSTπ)基因/蛋白表达的影响.方法 HepG2细胞43℃加热,每次80 min,2次/d,10个循环后,MTT法分析其对阿霉素的敏感性,免疫组化法检测细胞GSTπ蛋白的表达,RT-PCR法检测细胞GSTπmRNA的表达.结果热处理后的HepG2细胞对阿霉素的化疗耐受性升高,GSTπ基因/蛋白的表达增强.结论热处理后HepG2细胞对化疗药物的耐受性可能与其GSTπ基因/蛋白的表达增强有关.
목적 탐토다차가열후대잔존적간암HepG2세포적곡광감태-S-전이매π(GSTπ)기인/단백표체적영향.방법 HepG2세포43℃가열,매차80 min,2차/d,10개순배후,MTT법분석기대아매소적민감성,면역조화법검측세포GSTπ단백적표체,RT-PCR법검측세포GSTπmRNA적표체.결과 열처리후적HepG2세포대아매소적화료내수성승고,GSTπ기인/단백적표체증강.결론 열처리후HepG2세포대화료약물적내수성가능여기GSTπ기인/단백적표체증강유관.
Objective To investigate the expression change of ghtathione S-transferase π(GSTπ)protein and mRNA in the HepG2 cell after multiple thermotherapy.Methods HepG2 cells were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day，the sensibility of HepG2 cell to Adriamycin was analyzed by MTr assay，and the expression of QSTπprotein and mRNA were detected by immunohistochemical method and RT-PCR.Results The drug resistance to Adriamycin was gained by HepG2 cells after multiple thermotherapy，and the expression of GSTπ protein and mRNA wag strengthened.Conclusions The resistance of heated HepG2 cells to chemotherapeutics was concerned with overexpression of GSTπ protein and mRNA.