CAJ | 학술논문

目的探讨间质细胞telocytes在小鼠气管和肺组织中的分布特征.方法应用电镜、免疫组织化学、原代细胞培养及活细胞染色方法确定间质细胞telocytes在小鼠气管和肺组织中的分布特征.结果扫描及透射电镜均在小鼠气管软骨、平滑肌之间的间隙中找到了telocytes；在小鼠肺组织中,telocytes位于肺间质,紧邻毛细血管及小支气管.免疫组织化学检测发现telocytes表达c-kit、波形蛋白、CD34.从肺组织中分离培养得到的细胞具有典型的telocytes特征,即带有telopode的细胞,telopode也就是telocytes细胞胞体的延伸部分.透射电镜显示telopode呈管腔样结构,且厚薄交替,厚的部分称为podom,薄的部分称为podomer.结论 telocytes位于气管环状软骨和平滑肌之间的间隙中；在肺组织主要分布于小静脉、小支气管的间质中.
목적 탐토간질세포telocytes재소서기관화폐조직중적분포특정.방법 응용전경、면역조직화학、원대세포배양급활세포염색방법학정간질세포telocytes재소서기관화폐조직중적분포특정.결과 소묘급투사전경균재소서기관연골、평활기지간적간극중조도료telocytes；재소서폐조직중,telocytes위우폐간질,긴린모세혈관급소지기관.면역조직화학검측발현telocytes표체c-kit、파형단백、CD34.종폐조직중분리배양득도적세포구유전형적telocytes특정,즉대유telopode적세포,telopode야취시telocytes세포포체적연신부분.투사전경현시telopode정관강양결구,차후박교체,후적부분칭위podom,박적부분칭위podomer.결론 telocytes위우기관배상연골화평활기지간적간극중；재폐조직주요분포우소정맥、소지기관적간질중.
Objective To explore the location of telocytes in pulmonary tissues. Methods Electron microscopy,immunohistochemistry,primary cell culture and vital cell staining were used to identify the distribution of telocytes in mouse bronchial and pulmonary tissues.Results Telocytes were identified in the interstitial space between bronchial cartilage (cricoid) and smooth muscle by scanning and transmission electron microscope in mouse.By transmission electron micrscope and immunohistochemistry,telocytes were found in the interstitial spaces of lung parenchyma in connection with capillaries and bronchia.Telocytes expressed CD34, c-kit and vimetin by immunohistochemistry. After isolation, cultured telocytes demonstrated typical morphological feature,i.e.cells with telopode,which was seen as luminal structures with alternating thin and thick segments under electron microscope.Conclusion Telocytes are present in the interstitial space between cricoid cartilage and smooth muscle.