CAJ | 학술논문

目的从基因组全序列分析探讨浙江省1998-2009年H3N2亚型流感病毒的基因变异与流感流行的关系.方法选择浙江省1998-2009年流感流行期间分离到的H3N2亚型流感病毒流行代表株19株,采用RT-PCR方法扩增其8个基因片段后进行序列测定,并与近十余年间使用的疫苗株序列进行比较.结果 H3N2亚型流感流行株HA和NA基因的氨基酸变异率最高,分别为13.98%和10.00%;其他6个内部基因所表达的蛋白中,除NP、M2和NS1(氨基酸变异率分别为6.43%、6.19%和3.48%)外,其他蛋白的氨基酸变异均＜3.00%;在H3N2亚型流感存在较大范围流行的年份,除HA和NA基因外,流感病毒其他6个内部基因也会发生不同程度的变异.此外,在以往一些年份中,WHO推荐的H3N2流感病毒疫苗株与浙江省同期流行株之间,在许多基因上存在着滞后现象.结论在加强对H3N2亚型流感毒株表面HA与NA基因监测的同时,也应注意到其他内部基因的变异,为新一轮流感流行株的出现提供重要的预警信息.
목적 종기인조전서렬분석탐토절강성1998-2009년H3N2아형류감병독적기인변이여류감류행적관계.방법 선택절강성1998-2009년류감류행기간분리도적H3N2아형류감병독류행대표주19주,채용RT-PCR방법확증기8개기인편단후진행서렬측정,병여근십여년간사용적역묘주서렬진행비교.결과 H3N2아형류감류행주HA화NA기인적안기산변이솔최고,분별위13.98%화10.00%;기타6개내부기인소표체적단백중,제NP、M2화NS1(안기산변이솔분별위6.43%、6.19%화3.48%)외,기타단백적안기산변이균＜3.00%;재H3N2아형류감존재교대범위류행적년빈,제HA화NA기인외,류감병독기타6개내부기인야회발생불동정도적변이.차외,재이왕일사년빈중,WHO추천적H3N2류감병독역묘주여절강성동기류행주지간,재허다기인상존재착체후현상.결론 재가강대H3N2아형류감독주표면HA여NA기인감측적동시,야응주의도기타내부기인적변이,위신일륜류감류행주적출현제공중요적예경신식.
Objective To analyze the relationship between influenza epidemic and genetic characteristic on the whole genome of influenza virus subtype A/H3N2 strains isolated in Zhejiang province during 1998 to 2009. Methods All of the eight genes from the 19 Zhejiang influenza virus isolates, circulated during 1998 to 2009, were amplified by RT-PCR and sequenced. The obtained sequences were aligned and analyzed with the vaccine strains being used in the last 10 years.Results The highest mutation happened within HA and NA genes and the amino acid divergent ratios were 13.98% and 10.00%. Amongst the six internal proteins, the amino acid divergent ratios of NP, M2 and NS1 were 6.43%, 6.19% and 3.48% respectively, and the others were lower than 3%.Other than the HA and NA genes, mutations were also observed on six internal genes of the strains isolated in those years when the influenza virus subtype A/H3N2 was widely circulating.Additionally, there had been an obvious genetic lag between vaccine strains recommended by WHO and the contemporary Zhejiang epidemic strains for many years. Conclusion Besides on HA and NA genes, surveillance programs should also be covered mutations regarding the internal genes of influenza virus subtype A/H3N2 strains, in order to provide important information for forecasting and warning of a new round of influenza epidemic.