CAJ | 학술논문

目的观察急性淋巴细胞白血病(ALL)患者外周血中CD4+ CD25+调节性T细胞(Treg)的变化,探讨其临床意义.方法收集47例ALL初诊患者组、13例经化疗完全缓解组、9例未缓解组及20例健康对照组抗凝血,采用流式细胞仪检测CD4+ CD25+ Treg的水平.结果 CD4+ CD25+ Treg比例在ALL初诊组、治疗后完全缓解组、治疗后未缓解组和健康对照组分别为(5.78±2.42)％、(4.39±1.75)％、(6.76±2.57)％和(3.90±0.79)％.初诊组和未缓解组明显高于健康对照组(P =0.001,P=0.000),而完全缓解组与健康对照组比较无统计学意义(P =0.130)；化疗后完全缓解组和未缓解组与初诊组均无统计学意义(P=0.058,P=0.278),而完全缓解组明显低于未缓解组(P=0.018).结论 ALL患者外周血中高表达CD4+ CD25+ Treg可能是ALL免疫抑制的一个重要原因,其变化对于ALL的疗效评价及预后判断有一定的意义.
목적 관찰급성림파세포백혈병(ALL)환자외주혈중CD4+ CD25+조절성T세포(Treg)적변화,탐토기림상의의.방법 수집47례ALL초진환자조、13례경화료완전완해조、9례미완해조급20례건강대조조항응혈,채용류식세포의검측CD4+ CD25+ Treg적수평.결과 CD4+ CD25+ Treg비례재ALL초진조、치료후완전완해조、치료후미완해조화건강대조조분별위(5.78±2.42)％、(4.39±1.75)％、(6.76±2.57)％화(3.90±0.79)％.초진조화미완해조명현고우건강대조조(P =0.001,P=0.000),이완전완해조여건강대조조비교무통계학의의(P =0.130)；화료후완전완해조화미완해조여초진조균무통계학의의(P=0.058,P=0.278),이완전완해조명현저우미완해조(P=0.018).결론 ALL환자외주혈중고표체CD4+ CD25+ Treg가능시ALL면역억제적일개중요원인,기변화대우ALL적료효평개급예후판단유일정적의의.
Objective To observe CD4 + CD25 + regulatory T cells'level in peripheral blood of patients with acute lymphocytic leukemia and explore its clinical value.Methods Coagulated blood was collected from 4 groups people,including 47 ALL patients before chemotherapy,13 ALL patients with complete remission after chemotherapy,9 ALL patients without remission and 20 cases of healthy control.CD4 + CD25 + regulatory T ceils in peripheral blood were detected using flow cytometry.Results CD4 + CD25 + regulatory T cells'level in peripheral blood of ALL patients before chemotherapy,complete remission,without remission after chemotherapy and healthy control was (5.78 ±2.42)％、(4.39 ± 1.75)％、(6.76 ±2.57)％和(3.90 ±0.79)％ respectively.CD4 + CD25 + regulatory T cells' level of ALL patients before chemotherapy and without remission after chemotherapy was higher than that of healthy control( P =0.001,P =0.000).There was no significant difference between ALL patients with complete remission after chemotherapy and healthy control( P =0.130).Before and After chemotherapy,Whether remission or not,CD4+ CD25 + regulatory T cells'level had not the obviousdifference( P =0.058,P =0.278 ).But patients with complete remission was lower than whom without remission(p =0.018).Conclusions CD4 + CD25 + regulatory T cells' High-expression in peripheral blood of ALL patients is probably important in ALL immunological suppression.To a certain extent,it is valuable to assess healing effect and decide prognosis of ALL.