中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2011年
6期
405-409
,共5页
雷红召%董长宪%马玉春%孙斌%刘大看%肖莉
雷紅召%董長憲%馬玉春%孫斌%劉大看%肖莉
뢰홍소%동장헌%마옥춘%손빈%류대간%초리
成纤维细胞生长因子%受体%血管瘤%聚合酶链式反应
成纖維細胞生長因子%受體%血管瘤%聚閤酶鏈式反應
성섬유세포생장인자%수체%혈관류%취합매련식반응
Fibroblast growth factors%Receptor%Hemangiomas%Polymerase chain reaction
目的 通过检测不同时期血管瘤组织中碱性成纤维细胞生长因子(bFGF)及其受体(FGFR)的mRNA表达水平,初步探讨bFGF及其受体对血管瘤生长的影响,以进一步探讨血管瘤增生退化的机制.方法 取不同时期血管瘤组织及其邻近组织标本,其中增生期20例,退化期12例,正常皮肤组织8例作为对照.根据NCBI基因库中bFGF和FGFR的cDNA序列设计引物,以β-actin为内参照,应用逆转录聚合酶链式反(RT-PCR)方法半定量测定组织中bFGFmRNA和FGFRmRNA表达水平.并分析FGFRmRNA与bFGFmRNA表达的相关性.结论 琼脂糖凝胶电泳显示增生期血管瘤、退化期血管瘤、正常组织中bFGFmRNA和FGFRmRNA电泳条带亮度均依次降低;bFGFmRNA表达的相对量分别为增生期1.8495±0.0950、退化期1.3569±0.0417、正常组0.7188±0.0271;FGFRmRNA表达的相对量依次分别为增生期1.4858±0.1034、退化期0.9225±0.0532、正常组0.4144±0.0272;3组组织中bFGFmRNA和FGFRmRNA表达的相对量以增生期为最高,正常组最少.两组血管瘤组织与正常组织相比差异有显著统计学意义(P＜0.01),增生期与退化期之间比较差异亦有统计学意义(P＜0.01).经Pearson检验分析,FGFRmRNA与bFGFmRNA在增生期和退化期血管瘤组织中的表达具有显著正相关关系(r=0.850,P＜0.01;r=0.812,P＜0.01),在对照组亦呈正相关(r=0.804,P＜0.05).结论 bFGF及其受体在增生期血管瘤组织中高水平表达,在退化期中表达减弱,在正常组织中几乎无表达;FGFRmRNA与bFGFmRNA基因表达在3组组织中具有正相关关系,提示bFGF及其受体参与调控血管瘤的发展过程.
目的 通過檢測不同時期血管瘤組織中堿性成纖維細胞生長因子(bFGF)及其受體(FGFR)的mRNA錶達水平,初步探討bFGF及其受體對血管瘤生長的影響,以進一步探討血管瘤增生退化的機製.方法 取不同時期血管瘤組織及其鄰近組織標本,其中增生期20例,退化期12例,正常皮膚組織8例作為對照.根據NCBI基因庫中bFGF和FGFR的cDNA序列設計引物,以β-actin為內參照,應用逆轉錄聚閤酶鏈式反(RT-PCR)方法半定量測定組織中bFGFmRNA和FGFRmRNA錶達水平.併分析FGFRmRNA與bFGFmRNA錶達的相關性.結論 瓊脂糖凝膠電泳顯示增生期血管瘤、退化期血管瘤、正常組織中bFGFmRNA和FGFRmRNA電泳條帶亮度均依次降低;bFGFmRNA錶達的相對量分彆為增生期1.8495±0.0950、退化期1.3569±0.0417、正常組0.7188±0.0271;FGFRmRNA錶達的相對量依次分彆為增生期1.4858±0.1034、退化期0.9225±0.0532、正常組0.4144±0.0272;3組組織中bFGFmRNA和FGFRmRNA錶達的相對量以增生期為最高,正常組最少.兩組血管瘤組織與正常組織相比差異有顯著統計學意義(P＜0.01),增生期與退化期之間比較差異亦有統計學意義(P＜0.01).經Pearson檢驗分析,FGFRmRNA與bFGFmRNA在增生期和退化期血管瘤組織中的錶達具有顯著正相關關繫(r=0.850,P＜0.01;r=0.812,P＜0.01),在對照組亦呈正相關(r=0.804,P＜0.05).結論 bFGF及其受體在增生期血管瘤組織中高水平錶達,在退化期中錶達減弱,在正常組織中幾乎無錶達;FGFRmRNA與bFGFmRNA基因錶達在3組組織中具有正相關關繫,提示bFGF及其受體參與調控血管瘤的髮展過程.
목적 통과검측불동시기혈관류조직중감성성섬유세포생장인자(bFGF)급기수체(FGFR)적mRNA표체수평,초보탐토bFGF급기수체대혈관류생장적영향,이진일보탐토혈관류증생퇴화적궤제.방법 취불동시기혈관류조직급기린근조직표본,기중증생기20례,퇴화기12례,정상피부조직8례작위대조.근거NCBI기인고중bFGF화FGFR적cDNA서렬설계인물,이β-actin위내삼조,응용역전록취합매련식반(RT-PCR)방법반정량측정조직중bFGFmRNA화FGFRmRNA표체수평.병분석FGFRmRNA여bFGFmRNA표체적상관성.결론 경지당응효전영현시증생기혈관류、퇴화기혈관류、정상조직중bFGFmRNA화FGFRmRNA전영조대량도균의차강저;bFGFmRNA표체적상대량분별위증생기1.8495±0.0950、퇴화기1.3569±0.0417、정상조0.7188±0.0271;FGFRmRNA표체적상대량의차분별위증생기1.4858±0.1034、퇴화기0.9225±0.0532、정상조0.4144±0.0272;3조조직중bFGFmRNA화FGFRmRNA표체적상대량이증생기위최고,정상조최소.량조혈관류조직여정상조직상비차이유현저통계학의의(P＜0.01),증생기여퇴화기지간비교차이역유통계학의의(P＜0.01).경Pearson검험분석,FGFRmRNA여bFGFmRNA재증생기화퇴화기혈관류조직중적표체구유현저정상관관계(r=0.850,P＜0.01;r=0.812,P＜0.01),재대조조역정정상관(r=0.804,P＜0.05).결론 bFGF급기수체재증생기혈관류조직중고수평표체,재퇴화기중표체감약,재정상조직중궤호무표체;FGFRmRNA여bFGFmRNA기인표체재3조조직중구유정상관관계,제시bFGF급기수체삼여조공혈관류적발전과정.
Objective To investigate the expressions of basic fibroblast growth factor(bFGF) and its receptor(FGFR) in different phases of hemangiomas,to probe into the role of bFGF and FGFR in the development of hemangiomas,and to explore the mechanism of hemangiomatous proliferation and regression.Methods The fresh tissue from different phases of hemangiomas and theirs adjacent tissues were collected.The specimens including 20 proliferating phase,12 involuting phase hemangiomas,and 8 normal tissues as control group.According to the cDNA sequences of bFGF and FGFR in NCBI Gene Bank,the primers were designed and β-actin was used for internal competitive reference standard.The expressions of bFGF and FGFR mRNAs were examined by reverse transcriptase polymerase chain reaction(RT-PCR).The relation of FGFR mRNA and bFGF mRNA was analyzed by correlation analysis.Results The fluorescence brightness of bFGF mRNA and FGFR mRNA electrophoresis strap in the proliferating hemangioma,involuting hemangioma and normal skin was decreased in order.The relative expression of bFGF mRNA in tissues of proliferative hemangioma and involuting hemangioma was 1.8495±0.0950 and 1.3569±0.0417,which was significantly higher than that of normal skin(0.7188±0.0271).The relative expression of FGFR mRNA in the tissues of proliferative hemangioma,involuting hemangioma and normal skin was 1.4858±0.1034,0.9225±0.0532,and 0.4144±0.0272,respectively.By comparing the relative quantities of bFGF mRNA and FGFR mRNA,the highest expressions were in proliferating phase hemangiomas and the lowest were in normal skin.The relative expression quantities of bFGF mRNA and FGFR mRNA were obviously higher in hemangiomas than those in normal skin(P＜0.01).The relative quantities of bFGF mRNA and FGFR mRNA in hemangiomas were also higher at proliferative stage than those at degenerative stage(P＜0.01).There were significantly positive correlation between FGFR mRNA and bFGF mRNA in the tissue of proliferative stage hemangiomas and involuting stage hemangiomas(r=0.850,P＜0.01;r=0.812,P＜0.01).And it also showed positive correlation in control group(r=0.804,P＜0.05).Conclusions The expressions of bFGF and FGFR are increased in proliferating phase hemangioma,and decreased in involuting phase,but nearly blanked in control group.The positive correlation of FGFR mRNA and bFGF mRNA can be noted among proliferative hemangioma,involuting hemangioma or normal skin.
