中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
3期
226-228
,共3页
南娟%杜艳霞%银杰%赵永%张占雀%刘鹏%邢伟%蔡葵%蔡原%时利德
南娟%杜豔霞%銀傑%趙永%張佔雀%劉鵬%邢偉%蔡葵%蔡原%時利德
남연%두염하%은걸%조영%장점작%류붕%형위%채규%채원%시리덕
铝暴露%神经颗粒素%出生前后
鋁暴露%神經顆粒素%齣生前後
려폭로%신경과립소%출생전후
Aluminum exposure%Neurogranin%Period from prenatal to postnatal
目的 研究出生前后不同浓度慢性铝暴露对年轻大鼠海马神经颗粒素(Ng)的影响,进一步探讨铝损害学习与记忆的突触机制.方法 对照组、0.2%-Al组和0.4%-Al组大鼠各15只从孕期始分别自由饮用蒸馏水、Al3+ 浓度为15 mmol·L-1和30 mmol·L-1的A1C13水溶液制备出生前后慢性铝暴露模型;分别取学习和记忆的行为学测试后各组模型鼠9只,采用Western blot法检测海马细胞内Ng含量;组间资料统计用单因素方差分析,多重比较用LSD-t检验.结果 对照组大鼠海马Ng目标条带的平均密度值为(96.99±6.52);0.2%-Al暴露组为(83.59±7.17);0.4%-Al暴露组为(62.44±14.26).与对照组相比,0.2%-Al组海马Ng的表达明显降低( P =0.034),0.4%-Al组海马Ng表达显著性降低( P <0.01),且铝暴露组间亦差异有显著性( P =0.002).结论 出生前后慢性铝暴露使Ng含量降低是其导致学习与记忆功能下降的突触机制之一.
目的 研究齣生前後不同濃度慢性鋁暴露對年輕大鼠海馬神經顆粒素(Ng)的影響,進一步探討鋁損害學習與記憶的突觸機製.方法 對照組、0.2%-Al組和0.4%-Al組大鼠各15隻從孕期始分彆自由飲用蒸餾水、Al3+ 濃度為15 mmol·L-1和30 mmol·L-1的A1C13水溶液製備齣生前後慢性鋁暴露模型;分彆取學習和記憶的行為學測試後各組模型鼠9隻,採用Western blot法檢測海馬細胞內Ng含量;組間資料統計用單因素方差分析,多重比較用LSD-t檢驗.結果 對照組大鼠海馬Ng目標條帶的平均密度值為(96.99±6.52);0.2%-Al暴露組為(83.59±7.17);0.4%-Al暴露組為(62.44±14.26).與對照組相比,0.2%-Al組海馬Ng的錶達明顯降低( P =0.034),0.4%-Al組海馬Ng錶達顯著性降低( P <0.01),且鋁暴露組間亦差異有顯著性( P =0.002).結論 齣生前後慢性鋁暴露使Ng含量降低是其導緻學習與記憶功能下降的突觸機製之一.
목적 연구출생전후불동농도만성려폭로대년경대서해마신경과립소(Ng)적영향,진일보탐토려손해학습여기억적돌촉궤제.방법 대조조、0.2%-Al조화0.4%-Al조대서각15지종잉기시분별자유음용증류수、Al3+ 농도위15 mmol·L-1화30 mmol·L-1적A1C13수용액제비출생전후만성려폭로모형;분별취학습화기억적행위학측시후각조모형서9지,채용Western blot법검측해마세포내Ng함량;조간자료통계용단인소방차분석,다중비교용LSD-t검험.결과 대조조대서해마Ng목표조대적평균밀도치위(96.99±6.52);0.2%-Al폭로조위(83.59±7.17);0.4%-Al폭로조위(62.44±14.26).여대조조상비,0.2%-Al조해마Ng적표체명현강저( P =0.034),0.4%-Al조해마Ng표체현저성강저( P <0.01),차려폭로조간역차이유현저성( P =0.002).결론 출생전후만성려폭로사Ng함량강저시기도치학습여기억공능하강적돌촉궤제지일.
Objective To study the change of Ng in hippocampus of young rats after chronic aluminum exposure of different concentration from prenatal to postnatal stage,and to investigate the mechanism of aluminum-induced impairments to learning and memory. Methods Rats of control,low-dose and high-dose groups were treated from gestation with distiued water and AlCl3 water solution with concentration of 15 mmol·L-1 and 30 mmol·L-1 Al3+ respectively.The content of Ng was tested by Western-blot. Results The content of Ng in control group was 96.99±6.52,in 0.2%-Al group was 83.59±7.17,in 0.4%-Al group was 62.44±14.26.Compared with the control group,the content of Ng in 0.2%-Al group was remarkably decreased( P =0.034)and in 0.4%-Al group was significantly reduced( P <0.01),moreover,in the Al3+ exposed groups,the reduction was statistically significantly( P =0.002). Conclusion Aluminum exposure from prenatal to postnatal stage can reduce intracellular Ng content in hippocampus,which involve in impairing the ability of learning and memory.
