中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
8期
677-680
,共4页
彭永华%白云静%梁璟%郑希耕
彭永華%白雲靜%樑璟%鄭希耕
팽영화%백운정%량경%정희경
强迫性觅药%吗啡%行为敏感化%黑质网状部
彊迫性覓藥%嗎啡%行為敏感化%黑質網狀部
강박성멱약%마배%행위민감화%흑질망상부
Compulsive drug-seeking%Morphine%Addiction%Substantia nigra pars reticulate
目的 考察不同剂量吗啡用药模式下大鼠的行为敏感化表达是否受到前期用药环境的调控,探索行为敏感化表达不受前期用药环境影响的动物模型及其中脑区域神经元的活动性改变.方法 按吗啡给药方式将大鼠分为3组:累加大剂量吗啡前处理组(HD组)、固定小剂量吗啡前处理组(LD组)和盐水前处理组(S组).戒断后,在"非用药环境"中考察大鼠经吗啡点燃后的水平运动和刻板运动.随后进行腹侧被盖区(VTA)和黑质(SN)的Fos蛋白和酪氨酸羟化酶(Tyrosine hydroxylase,TH)双重免疫组织化学染色.结果 10mg/kg吗啡点燃后,HD组水平运动和刻板运动均显著高于S组(水平运动和刻板运动,HD组:26907±2003和129.6±6.5;S组:14962±1888和89.9±10.9,均P＜0.01).与S组比较,HD组在SNr整体及SNr外侧部Fos蛋白阳性神经元均显著增高(SNr和SNr外侧部Fos阳性神经元,HD组:29.14±5.27和9.83±2.84;S组:17.29±1.51和2.06±0.45;均P＜0.05).结论 相对于固定小剂量吗啡用药,累加大剂量吗啡用药诱导的大鼠行为敏感化不受前期用药环境调控;SNr尤其是其外侧部的神经元活动性增加与累加大剂量吗啡用药大鼠的觅药动机表达有重要关系.
目的 攷察不同劑量嗎啡用藥模式下大鼠的行為敏感化錶達是否受到前期用藥環境的調控,探索行為敏感化錶達不受前期用藥環境影響的動物模型及其中腦區域神經元的活動性改變.方法 按嗎啡給藥方式將大鼠分為3組:纍加大劑量嗎啡前處理組(HD組)、固定小劑量嗎啡前處理組(LD組)和鹽水前處理組(S組).戒斷後,在"非用藥環境"中攷察大鼠經嗎啡點燃後的水平運動和刻闆運動.隨後進行腹側被蓋區(VTA)和黑質(SN)的Fos蛋白和酪氨痠羥化酶(Tyrosine hydroxylase,TH)雙重免疫組織化學染色.結果 10mg/kg嗎啡點燃後,HD組水平運動和刻闆運動均顯著高于S組(水平運動和刻闆運動,HD組:26907±2003和129.6±6.5;S組:14962±1888和89.9±10.9,均P＜0.01).與S組比較,HD組在SNr整體及SNr外側部Fos蛋白暘性神經元均顯著增高(SNr和SNr外側部Fos暘性神經元,HD組:29.14±5.27和9.83±2.84;S組:17.29±1.51和2.06±0.45;均P＜0.05).結論 相對于固定小劑量嗎啡用藥,纍加大劑量嗎啡用藥誘導的大鼠行為敏感化不受前期用藥環境調控;SNr尤其是其外側部的神經元活動性增加與纍加大劑量嗎啡用藥大鼠的覓藥動機錶達有重要關繫.
목적 고찰불동제량마배용약모식하대서적행위민감화표체시부수도전기용약배경적조공,탐색행위민감화표체불수전기용약배경영향적동물모형급기중뇌구역신경원적활동성개변.방법 안마배급약방식장대서분위3조:루가대제량마배전처리조(HD조)、고정소제량마배전처리조(LD조)화염수전처리조(S조).계단후,재"비용약배경"중고찰대서경마배점연후적수평운동화각판운동.수후진행복측피개구(VTA)화흑질(SN)적Fos단백화락안산간화매(Tyrosine hydroxylase,TH)쌍중면역조직화학염색.결과 10mg/kg마배점연후,HD조수평운동화각판운동균현저고우S조(수평운동화각판운동,HD조:26907±2003화129.6±6.5;S조:14962±1888화89.9±10.9,균P＜0.01).여S조비교,HD조재SNr정체급SNr외측부Fos단백양성신경원균현저증고(SNr화SNr외측부Fos양성신경원,HD조:29.14±5.27화9.83±2.84;S조:17.29±1.51화2.06±0.45;균P＜0.05).결론 상대우고정소제량마배용약,루가대제량마배용약유도적대서행위민감화불수전기용약배경조공;SNr우기시기외측부적신경원활동성증가여루가대제량마배용약대서적멱약동궤표체유중요관계.
Objective To probe the different expression of psychomotor in different model of pretreatment and the influence of pretreatment context on the expression of psychomotor.And to compare dopaminergic and non-dopaminergic neuron activity in midbrain after different pretreatment.Methods Rats were pretreated by escalated high-dose morphine( HD group) ,fixed low-dose morphine( LD group) and saline as control(S group).After withdrawal, rats were challenged by morphine in non-drug used environment.Locomotor behavior and stereotypy behavior were monitored as the index of compulsive drug-seeking motivation.By double labeling immunohistochemistry of tyrosine hydroxylase (TH) and Fos protein, the plasticity of neuronal activity in ventral tegmental area and substantia nigra of rats response to morphine challenge were disclose after pretreated with morphine or saline.Results After 10 mg/kg morphine challenge in non-drug used environment,the locomotor behavior and the stereotypy behavior of HD group were both significant higher than that of S group ( Locomotor behavior and Stereotypy behavior,HD group: 26907 ± 2003 and 129.6 ± 6.5; S group: 14962 ± 1888 and 89.9 ± 10.9, all P ＜ 0.01 ).The results of immunohistochemistry showed that Fos protein expressed in the SNr and in lateral SNr was significantly higher in the HD group compared to S groups ( Fos expression in SNr and lateral SNr,HD group: 29.14 ±5.27 and 9.83 ± 2.84; S group: 17.29 ± 1.51 and 2.06 ±0.45; all P＜0.05 ).Conclusion The sensitization behavior expression of the rat pretreated by escalated high-dose morphine is not modulated by drug using enviroment compared with fixed low-dose morphine pretreatment.The neuroplasticity of SNr,especially lateral SNr might be one of the mechanisms underlying the uncontroled sensitization behavior expression.
