CAJ | 학술논문

目的分析HIV-1毒株vpr基因变异规律.方法 RT-PCR扩增HIV-1毒株vpr基因,经测序后构建基因进化树并分析其亚型分布;比较Vpr蛋白32～46位关键肽段氨基酸序列的差异;分析国内外Vpr蛋白77位氨基酸多态性的分布差异.结果 01_AE为HIV-1主要亚型(49.53%);B亚型vpr基因组内变异最大;Vpr蛋白77位存在多态性,分别编码谷氨酰胺、精氨酸和组氨酸残基,这种多态性在国内外分布差异无统计学意义(P=0.617).结论 HIV-1毒株vpr基因分型以01_AE亚型为主,vpr基因存在多态性,与国内外报道一致.
목적 분석HIV-1독주vpr기인변이규률.방법 RT-PCR확증HIV-1독주vpr기인,경측서후구건기인진화수병분석기아형분포;비교Vpr단백32～46위관건태단안기산서렬적차이;분석국내외Vpr단백77위안기산다태성적분포차이.결과 01_AE위HIV-1주요아형(49.53%);B아형vpr기인조내변이최대;Vpr단백77위존재다태성,분별편마곡안선알、정안산화조안산잔기,저충다태성재국내외분포차이무통계학의의(P=0.617).결론 HIV-1독주vpr기인분형이01_AE아형위주,vpr기인존재다태성,여국내외보도일치.
Objective To understand the rule of vpr gene variance of HIV-1 strains.Methods RT-PCR was used to amplify vpr gene of HIV-1 strains in Shenzhen. PCR products were sequenced and used for gene phylogenetic analysis and the 32-46 amino acids of Vpr protein were compared. The difference of 77 amino acid polymorphism distribution between domestic region and foreign region was analyzed. Results 01_AE was the major HIV-1 subtype in Shenzhen. The gene distance among subtype B was larger than in other subtypes. 77-amino acid of Vpr protein had three polymorphism forms as Arginin, Glutamine and Histidine, with Glutamine as the wild form. There were no significant differences in the three amino acid distributions between HIV-1 strains from domestic region and foreign region. Conclusion vpr genes of different HIV-1 strains belonged to 01_AE subtype. There was polymorphism seen in the vpr gene which was consistent with both domestic and international HIV-1 strains.