CAJ | 학술논문

目的探讨Cdk5的激活剂-p39mRNA在脑发育过程中的不同表达.方法用Northern blot法和原位杂交法检测p39mRNA在大鼠脑发育过程中的表达.结果 Northern blot结果显示,在15 d胚胎和新生大鼠脑中,p39mRNA表达较低;出生后1～3周表达最显著;到成年大鼠,表达降低到与新生大鼠脑相同的水平.原位杂交法显示,p39mRNA在新生大鼠脑中所有区域神经元中表达较低;而在3周龄大鼠脑的所有区域表达最高;到成年大鼠,大多数神经元中表达都降低,仅小脑还保持着高水平的表达.结论大鼠脑发育过程中,p39 mRNA在空间上和时间上有不同的表达,其亚细胞定位可能与激活Cdk5功能有关.
목적 탐토Cdk5적격활제-p39mRNA재뇌발육과정중적불동표체.방법 용Northern blot법화원위잡교법검측p39mRNA재대서뇌발육과정중적표체.결과 Northern blot결과 현시,재15 d배태화신생대서뇌중,p39mRNA표체교저;출생후1～3주표체최현저;도성년대서,표체강저도여신생대서뇌상동적수평.원위잡교법현시,p39mRNA재신생대서뇌중소유구역신경원중표체교저;이재3주령대서뇌적소유구역표체최고;도성년대서,대다수신경원중표체도강저,부소뇌환보지착고수평적표체.결론대서뇌발육과정중,p39 mRNA재공간상화시간상유불동적표체,기아세포정위가능여격활Cdk5공능유관.
Objective To detect the different distribution of Cdk5 two activators, p35 and p39, throughout brain development. Methods We examined the spatial and temporal expression of p39 in the developing rat brains by Northern blot analysis and in situ hybridization. Results Northern blot analysis showed that p39 expression was low in 15-day old fetuses and newborns, and was most prominent in the 1-3 week-old rat brains. In the adult rat brains, expression declined to the same level as in newborn rat brains. In situ hybridization showed that p39 mRNA was weakly expressed in all neurons of all regions in the newborn rat brains and the transcriptional level was highest in all regions in the 3 week-old rat brains. In the adult rat brains, the expression was decreased in most neurons, except Purkinje and granule cells in the cerebellum which retained high levels. Conclusion The expression of p39 varies spatially and temporally along with rat brain development, and its subcellular location is related with the activation of Cdk5 function.