CAJ | 학술논문

目的观察siRNA下调Caveolin-1(Car1)表达的效果及对体外培养C6胶质瘤细胞侵袭和迁移能力的影响.方法构建Car1 mRNA质粒,转染体外培养c6胶质瘤细胞,利用Transwell体外侵袭实验和"划痕"实验分别检测细胞的侵袭和迁移能力,采用Western blot和RT-PCR的方法检测Cav1、EGFR、Pyk2的表达情况.结果所构建的Cav1 siRNA质粒转染体外培养C6胶质瘤细胞可明显下调Cav1的表达,干扰成功后C6胶质瘤细胞的侵袭能力和迁移能力明显下降,同时EGFR和Pyk2的表达明显减少.结论 siRNA下调Cav1可降低体外培养C6胶质瘤细胞的侵袭和迁移能力.
목적 관찰siRNA하조Caveolin-1(Car1)표체적효과급대체외배양C6효질류세포침습화천이능력적영향.방법 구건Car1 mRNA질립,전염체외배양c6효질류세포,이용Transwell체외침습실험화"화흔"실험분별검측세포적침습화천이능력,채용Western blot화RT-PCR적방법검측Cav1、EGFR、Pyk2적표체정황.결과 소구건적Cav1 siRNA질립전염체외배양C6효질류세포가명현하조Cav1적표체,간우성공후C6효질류세포적침습능력화천이능력명현하강,동시EGFR화Pyk2적표체명현감소.결론 siRNA하조Cav1가강저체외배양C6효질류세포적침습화천이능력.
Objective To study the in vitro migration and invasion of C6 glioma cell after Caveolin -1 (Car1) knocking- down by siRNA. Methods Car1 siRNA plasmids were transfected to C6 glioma cells. Abilities of cell invasion and migration were examined by Transwell and "Wound - healing" methods respectively. Expressions of eaveolin-1, EGFR, Pyk2 were measured by Western blot and RT - PCR. Results The siRNA plasmid used in this experiment can obviously knock - down the expression of Car1. The invasion and the migration ability of C6 glioma cell decreased after siRNA as well as the expression of EGFR and Pyk2. Conclusions Carl Knocking - down results in the decreasing of in vitro invasion and migration of C6 glioma. It maybe a potential target for anti - invasion therapy on gliomas.