分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2001年
1期
5-7
,共3页
高效液相色谱法%蒸发光散射检测法%海藻糖
高效液相色譜法%蒸髮光散射檢測法%海藻糖
고효액상색보법%증발광산사검측법%해조당
用反相高效液相色谱法分离-蒸发光散射检测法测定了海藻糖的纯度，检测下限为 5 mg/L。在 30～1000 mg/L 范围内，海藻糖浓度的对数与 ELSD 测得的峰面积对数具有良好的线性关系。
用反相高效液相色譜法分離-蒸髮光散射檢測法測定瞭海藻糖的純度，檢測下限為 5 mg/L。在 30～1000 mg/L 範圍內，海藻糖濃度的對數與 ELSD 測得的峰麵積對數具有良好的線性關繫。
용반상고효액상색보법분리-증발광산사검측법측정료해조당적순도，검측하한위 5 mg/L。재 30～1000 mg/L 범위내，해조당농도적대수여 ELSD 측득적봉면적대수구유량호적선성관계。
A reversed-phase high performance chromatography with evaporative light-scattering detector (ELSD) using methanol-water (4∶1,v/v) as mobile phase was established for direct purity examination of trehalose. An ODS column Zorbax C18 was employed. Regression equations reveal linear relationship between logarithm of trehalose concentration and logarithm of trehalose peak area detected by ELSD in the range of 30～1000 mg/L trehalose. Recoveriy of trehalose measured by ELSD is 99.42% with detection limit of 5 mg/L.
