CAJ | 학술논문

牛分枝杆菌是引起牛结核病(bTB)的病原体,侵染宿主广泛,可感染多种家畜和野生动物,对人类和动物健康构成巨大危害.随着多年不懈的研究,有关分枝杆菌分子流行病学的知识积累不断增加.作者对近年来应用新的基因分型技术研究牛结核分枝杆菌分子流行病学方面的一些研究进展.这些技术包限制性片段长度多态性分析(RFLP)、PCR介导的间隔区寡核苷酸分析(spoligotyping)、数目可变串联重复位点(VNTR)等.主要用于对人、牛、家畜以及野生动物的结核病分子流行病学进行分析和监测.另外,还利用一系列敏感的基于PCR的技术从分枝杆菌杆菌复合体(NTM)中对菌型进行鉴别.对分枝杆菌感染分子流行病学的全面了解,有助于对本病扩散传播机制的深入研究.可为制定科学防治结核病的措施做出贡献.
우분지간균시인기우결핵병(bTB)적병원체,침염숙주엄범,가감염다충가축화야생동물,대인류화동물건강구성거대위해.수착다년불해적연구,유관분지간균분자류행병학적지식적루불단증가.작자대근년래응용신적기인분형기술연구우결핵분지간균분자류행병학방면적일사연구진전.저사기술포한제성편단장도다태성분석(RFLP)、PCR개도적간격구과핵감산분석(spoligotyping)、수목가변천련중복위점(VNTR)등.주요용우대인、우、가축이급야생동물적결핵병분자류행병학진행분석화감측.령외,환이용일계렬민감적기우PCR적기술종분지간균간균복합체(NTM)중대균형진행감별.대분지간균감염분자류행병학적전면료해,유조우대본병확산전파궤제적심입연구.가위제정과학방치결핵병적조시주출공헌.
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), has a wide host range, infecting many domestic and wild animals and is one of a potential health hazard for animals and humans. With the research being continued in recent years, the knowledge of Molecular epidemiology of the infection have been increasing. This review discusses the currently available information on Molecular epidemiology of M. Bovis infection analyzed by the usefulness of new genotyping techniques. Such as carrying out the unequivocal differential detection of M. Bovis and M. Tuberculosis with restriction fragment length polymorphisms (RFLP); applying PCR-based spoligotyping and variable -number tandem repeat (VNTR) assay for epidemiological surveillance M. Bovis infection in human, cattle and other wild or domestic animals, and evolution oftubereulosis pathogens; using a series of sensitive PCR methods to discriminate one species from non-tuberculous mycobacteria (NTM) A complete understanding of the Molecular epidemiology of M. Bovis infection could give important insights into the extent and spread of disease, which will contribute to the development of ideal control schemes of M. Bovis infections.