CAJ | 학술논문

指数级富集的配体系统进化技术(SELEX)是近年来发展的获得能够与靶分子高特异性和高亲力结合的寡核苷酸序列(适配体)的筛选技术.目前多种靶分子的适配体如蛋白或小分子,都已经通过SELEX技术筛选获得,使适配体在蛋白质组研究、临床医学、药物研发及基因调控等领域已经成为重要的研究工具.本文就近几年适配体的筛选技术及在生命分析化学中的应用发展方面进行了综述.
지수급부집적배체계통진화기술(SELEX)시근년래발전적획득능구여파분자고특이성화고친력결합적과핵감산서렬(괄배체)적사선기술.목전다충파분자적괄배체여단백혹소분자,도이경통과SELEX기술사선획득,사괄배체재단백질조연구、림상의학、약물연발급기인조공등영역이경성위중요적연구공구.본문취근궤년괄배체적사선기술급재생명분석화학중적응용발전방면진행료종술.
The systematic evolution of ligands by experimental enrichment(SELEX) process has been developed for isolation of oligonucleotide sequences(referred as aptamers) with the capacity to recognize various classes of target molecules with high affinity and specificity.Various analytical apatmers have been used as suitable tools for detection and quantification of target molecules.In this reviews,the selection and the application in biological analytical chemistry of aptamer are addressed.