CAJ | 학술논문

用强度为30 μW/cm~2、60 μW/cm~2、90 μW/cm~2、120 μW/cm~2、150 μW/cm~2的中波红斑效应紫外线(UVB)分别照射小鼠脾淋巴细胞5 min、15 min、30 min,采用单细胞凝胶电泳法(SCGE)检测UVB照射对细胞DNA的损伤,结果照射5 min和15 min时,DNA损伤程度与照射强度呈正相关;而照射30 min时,30 μW/cm~2组DNA损伤程度最高,60 μW/cm~2、90 μW/cm~2、120 μW/cm~2组损伤程度有所降低,而150 μW/cm~2组又出现了高DNA损伤.认为通过SCGE来检测UVB照射对小鼠脾淋巴细胞的损伤程度,可广泛应用于环境毒理学中对DNA具有损伤作用的分析,为环境生物学研究辐射对生物体的影响提供借鉴.
용강도위30 μW/cm~2、60 μW/cm~2、90 μW/cm~2、120 μW/cm~2、150 μW/cm~2적중파홍반효응자외선(UVB)분별조사소서비림파세포5 min、15 min、30 min,채용단세포응효전영법(SCGE)검측UVB조사대세포DNA적손상,결과조사5 min화15 min시,DNA손상정도여조사강도정정상관;이조사30 min시,30 μW/cm~2조DNA손상정도최고,60 μW/cm~2、90 μW/cm~2、120 μW/cm~2조손상정도유소강저,이150 μW/cm~2조우출현료고DNA손상.인위통과SCGE래검측UVB조사대소서비림파세포적손상정도,가엄범응용우배경독이학중대DNA구유손상작용적분석,위배경생물학연구복사대생물체적영향제공차감.
In this study, various intensities (30 μW/cm~2, 60 μW/cm~2, 90 μW/cm~2, 120 μW/cm~2, 150 μW/cm~2) of UV-B were used to irradiate mouse spleen lymphocytes for 5 min, 15 min, and 30 min. Then single-cell gel electrophoresis (SCGE) was utilized to detect the damage grade of DNA in spleen lymphocytes. The results showed that high intensity UV-B irradiation led to severe DNA damage of spleen lymphocytes after 5 min and 15 min's irradiation; however, when the irradiation time was 30 min, the DNA damage grade was the highest at 30 μW/cm~2 and it decreased from 60 μW/cm~2 to 120 μW/cm~2, then it came to another peak at 150 μW/cm~2. Here we adopt SCGE to detect DNA damage after irradiation of spleen lymphocytes by UV-B, which will be widely used to analyze DNA damage in environmental toxicology and offer a reference to study irradiation effects in environmental biology.