细胞与分子免疫学杂志
細胞與分子免疫學雜誌
세포여분자면역학잡지
2009年
10期
935-937
,共3页
傅颖珺%袁娟丽%陈江%谢勇
傅穎珺%袁娟麗%陳江%謝勇
부영군%원연려%진강%사용
连翘%CD4+CD25+调节性T细胞(Treg)%Foxp3
連翹%CD4+CD25+調節性T細胞(Treg)%Foxp3
련교%CD4+CD25+조절성T세포(Treg)%Foxp3
Forsythia suspense%CD4~+ CD25~+ regulatory T cell%Foxp3
目的:探讨中药连翘对严重烧伤大鼠的免疫调节作用及其机制.方法:采用大鼠30%体表面积Ⅲ度烧伤模型.实验共分5组,分别为正常对照组(Control),烧伤后8 h组(8PBH),连翘高、中、低剂量组(AFS1、AFS2、AFS3),其中AFS1、AFS2和AFS3组在烧伤前7 d开始灌胃给药,1次/d,分别给予AFS 5 g/kg、2.5 g/kg和1.25 g/kg.各组在烧伤后8 h留取外周血和脾脏组织,流式细胞术测外周血Treg百分数;RT-PCR和免疫组化法测定脾脏组织Foxp3表达.结果:与Control组比较,8PBH组外周血Treg水平显著升高,脾脏组织Foxp3mRNA和蛋白表达明显上调;AFS1、AFS2及APS3组均能显著减轻上述指标变化,并呈剂量依赖关系.结论:连翘具有免疫调节作用,其作用机制与干扰Foxp3基因的表达有关.
目的:探討中藥連翹對嚴重燒傷大鼠的免疫調節作用及其機製.方法:採用大鼠30%體錶麵積Ⅲ度燒傷模型.實驗共分5組,分彆為正常對照組(Control),燒傷後8 h組(8PBH),連翹高、中、低劑量組(AFS1、AFS2、AFS3),其中AFS1、AFS2和AFS3組在燒傷前7 d開始灌胃給藥,1次/d,分彆給予AFS 5 g/kg、2.5 g/kg和1.25 g/kg.各組在燒傷後8 h留取外週血和脾髒組織,流式細胞術測外週血Treg百分數;RT-PCR和免疫組化法測定脾髒組織Foxp3錶達.結果:與Control組比較,8PBH組外週血Treg水平顯著升高,脾髒組織Foxp3mRNA和蛋白錶達明顯上調;AFS1、AFS2及APS3組均能顯著減輕上述指標變化,併呈劑量依賴關繫.結論:連翹具有免疫調節作用,其作用機製與榦擾Foxp3基因的錶達有關.
목적:탐토중약련교대엄중소상대서적면역조절작용급기궤제.방법:채용대서30%체표면적Ⅲ도소상모형.실험공분5조,분별위정상대조조(Control),소상후8 h조(8PBH),련교고、중、저제량조(AFS1、AFS2、AFS3),기중AFS1、AFS2화AFS3조재소상전7 d개시관위급약,1차/d,분별급여AFS 5 g/kg、2.5 g/kg화1.25 g/kg.각조재소상후8 h류취외주혈화비장조직,류식세포술측외주혈Treg백분수;RT-PCR화면역조화법측정비장조직Foxp3표체.결과:여Control조비교,8PBH조외주혈Treg수평현저승고,비장조직Foxp3mRNA화단백표체명현상조;AFS1、AFS2급APS3조균능현저감경상술지표변화,병정제량의뢰관계.결론:련교구유면역조절작용,기작용궤제여간우Foxp3기인적표체유관.
AIM: To explore the immunity modulation function of aqueous of Forsythia suspense (AFS) and its possible mechanisms. METHODS: Rats of burned model group were burned with vapor under 3mpa pressure and 108℃ temperature for 8 seconds to achieve deep partialthickness bum, to make a thirty percent total body surface area (TBSA)bum. The experiment were divided into five groups: Control group: without any treatment; 8 PBH group: 8 h after burn; the rats of AFS1 guoup, AFS2 group and AFS3 group of them were given AFS 5 g/kg, 2.5 g/kg, 1.25 g/kg once a day by Po. pathway for seven days before burns, respectively. Rats were sacrificed before and 8h after burn, The percentage of Treg cells in CD4~+ T cells was detected by flow cytometry(FCM) ; the expression of Foxp3 mRNA on splenocytes were measured by RT-PCR, and the protein of Foxp3 activity was evaluated by immunohistochemistry staining. RESULTS: Compared with Control group, the expression of Foxp3mRNA and protein on the splenocytes were upregulated markedly(P <0.01), and the percentage of Treg were significantly increased (P < 0.01) in the 8PBH group. AFS1, AFS2 and AFS3 significantly attenuated these increases (P < 0.01), which was dose-dependent. CONCLUSION: AFS has immunity modulation function and mechanism of it is corrected with Foxp3 mRNA on splenocytes.