CAJ | 학술논문

以山薯无菌苗茎段为试验材料,研究BA、NAA、JA、蔗糖浓度、光周期以及不同培养方式对试管零余子诱导形成的影响.结果表明,MS+2.0mg/L BA+0.1 mg,L NAA+80g/L蔗糖+5.0g/L,琼脂+1.5 g/L活性炭,12 h/d的光照时间(光照强度为2 000 lx)是试管零余子诱导较优的培养条件.经过2个月的培养,试管零余子诱导率达90%以上,零余子发生个数为1.44个/株.蔗糖浓度是影响试管零余子发生的最重要因素,高蔗糖浓度(80 g/L)能促进试管零余子的发生.
이산서무균묘경단위시험재료,연구BA、NAA、JA、자당농도、광주기이급불동배양방식대시관령여자유도형성적영향.결과표명,MS+2.0mg/L BA+0.1 mg,L NAA+80g/L자당+5.0g/L,경지+1.5 g/L활성탄,12 h/d적광조시간(광조강도위2 000 lx)시시관령여자유도교우적배양조건.경과2개월적배양,시관령여자유도솔체90%이상,령여자발생개수위1.44개/주.자당농도시영향시관령여자발생적최중요인소,고자당농도(80 g/L)능촉진시관령여자적발생.
Clean stem segments of Dioscorea fordii Prain et Burk were used as in vitro microcutting materials to induce formation of bulbils under mediums in the presence of various levels of BA, NAA, JA, sucrose and photoperiod. The results showed that the preferred condition for induction of in vitro bulbils was MS basal medium supplemented with 2.0 mg/L BA, 0.1 mg/L NAA, 80 g/L sucrose, 1.5 g/L activated coal and 5.0 g/L agar under a 12/12 h/h(day/night) photoperiod. For 2 months of culture, the stem segments had an in vitro bulbils inducing frequency of above 90% , and produced 1.44 bulbils/plantlet. The concentration of sucrose was the most important factor which affected the induction of ire vitro bulbils, and higher concentration of sucrose(80 g/L) promoted the formation of in vitro bulbils.