CAJ | 학술논문

提出一种高灵敏的、基于银增强放大和免疫胶体金技术压电石英晶体免疫传感器.采用夹心法模式,在电极表面固定日本血吸虫抗原,用以捕获血清中的日本血吸虫兔抗血吸虫抗体,再与胶体金标记的羊抗兔IgG二抗形成免疫复合物,加入银增强溶液,通过胶体金催化银离子还原,使大量银沉积在纳米金周围,提高压电石英晶体免疫传感器的灵敏度.结果表明,基于胶体金标记银增强的日本血吸虫压电石英晶体免疫传感器,对兔抗血吸虫抗体具有较高的识别能力,其操作简单、灵敏度高、线性范围宽、检测下限低.将该方法应用于兔血清中日本血吸虫抗体的测定,取得了满意结果.
제출일충고령민적、기우은증강방대화면역효체금기술압전석영정체면역전감기.채용협심법모식,재전겁표면고정일본혈흡충항원,용이포획혈청중적일본혈흡충토항혈흡충항체,재여효체금표기적양항토IgG이항형성면역복합물,가입은증강용액,통과효체금최화은리자환원,사대량은침적재납미금주위,제고압전석영정체면역전감기적령민도.결과표명,기우효체금표기은증강적일본혈흡충압전석영정체면역전감기,대토항혈흡충항체구유교고적식별능력,기조작간단、령민도고、선성범위관、검측하한저.장해방법응용우토혈청중일본혈흡충항체적측정,취득료만의결과.
A high-sensitivity piezoelectric quartz crystal immunosensor based on silver enhancement and immuno-colloidal gold technique was developed.Using the sandwich method,the Schistosoma japonicum antigen was fixed in the electrode surface for capturing the Schistosoma japonicum antibody,which then was conjugated with the secondary antibody of goat anti-rabbit IgG labeled marked colloidal gold.Through the reaction catalyzed by colloidal gold upper silver ions reduction,a large number of silver deposited on the nano-around gold to enhance the sensitivity of piezoelectric quartz crystal immunosensor.The results show that the piezoelectric quartz crystal immunosensor based on colloidal gold labeling and silver enhancement is simple to operate,with high sensitivity,wide linear range,and low detection limit.The method has been applied to the rabbit serum antibodies in the determination of Schistosoma japonicum,and satisfactory results have been achieved.