家禽科学
傢禽科學
가금과학
POULTRY SCIENCE
2011年
10期
10-13
,共4页
于可响%沙明利%林树乾%姜亦飞%黄兵
于可響%沙明利%林樹乾%薑亦飛%黃兵
우가향%사명리%림수건%강역비%황병
1型鸭肝炎病毒%RT—PCR%全长cDNA克隆
1型鴨肝炎病毒%RT—PCR%全長cDNA剋隆
1형압간염병독%RT—PCR%전장cDNA극륭
duck hepatitis virus （DHV）typel%reverse transcription PCR%the full-length clone. cDNA
参考1型鸭肝炎病毒基因组序列，设计7条引物，用RT—PCR方法扩增出覆盖整个病毒基因组三个忠实性片段，并按顺序组装进载体pBR322中，获得全长cDNA克隆。测序结果表明，该克隆与母本毒序列同源性达99．6％，并且5’端的T7启动子和3’端的Mlu I线性化位点均成功引入。
參攷1型鴨肝炎病毒基因組序列，設計7條引物，用RT—PCR方法擴增齣覆蓋整箇病毒基因組三箇忠實性片段，併按順序組裝進載體pBR322中，穫得全長cDNA剋隆。測序結果錶明，該剋隆與母本毒序列同源性達99．6％，併且5’耑的T7啟動子和3’耑的Mlu I線性化位點均成功引入。
삼고1형압간염병독기인조서렬，설계7조인물，용RT—PCR방법확증출복개정개병독기인조삼개충실성편단，병안순서조장진재체pBR322중，획득전장cDNA극륭。측서결과표명，해극륭여모본독서렬동원성체99．6％，병차5’단적T7계동자화3’단적Mlu I선성화위점균성공인입。
Seven primers were synthetized according to genome sequence of duck hepatitis virus （DHV）typel, and three fidelity were amplified by RT-PCR, and these DNA fragments covering the full genome of DHV typel fragments were inserted into pBR322 vector according to the genome sequence, then gained the full-length cDNA clone.Sequence homology is 99.6% with the clone and female parent virus, and T7 promotor and Mlu I site are respectively added to genome 5＇ end and 3＇ end.
