CAJ | 학술논문

目的:阐明神经钙黏蛋白(N-cadherin)在神经嵴细胞分层中的作用.方法:利用原位杂交鉴定N-cadherin在神经管上的表达.转染过表达N-cadherin的野生型N-cadherin(wild-type N-cadherin,wt-N-cad)或阻断N-cadherin表达的结构域缺失型N-cadherin(dominant-negative N-cadherin,dn-N-cad),利用免疫组化方法检测头部神经嵴细胞的迁移变化.结果:无论是过表达N-cadherin还是下调N-cadherin表达,头部神经嵴细胞迁移都受到了影响.结论:头部神经嵴细胞的分层和迁移依赖于神经管上N-cadherin的表达.
목적:천명신경개점단백(N-cadherin)재신경척세포분층중적작용.방법:이용원위잡교감정N-cadherin재신경관상적표체.전염과표체N-cadherin적야생형N-cadherin(wild-type N-cadherin,wt-N-cad)혹조단N-cadherin표체적결구역결실형N-cadherin(dominant-negative N-cadherin,dn-N-cad),이용면역조화방법검측두부신경척세포적천이변화.결과:무론시과표체N-cadherin환시하조N-cadherin표체,두부신경척세포천이도수도료영향.결론:두부신경척세포적분층화천이의뢰우신경관상N-cadherin적표체.
AIM: To investigate the role of N-cadherin in the delamination of neural crest cells. METHODS: The normal expression of N-cadherin in neural tube was identified using in situ hybridization. The cells with N-cadherin over expression were obtained by transfection of wild-type N-cadherin (wt-N-cadherin) ,and the cells with N-cadherin silencing expression were obtained by transfection of dominant-negative N-cadherin (dn-N-cadherin). The migration of cranial neural crest cells was determined by the technique of immunohistochemistry. RESULTS: Either overexpression or down-regulation of N-cadherin significantly affected the migration of cranial neural crest cells. CONCLUSION: Delamination and migration of the cranial neural crest cells rely on the relative N-cadherin expression in the neural tube during neurulation.