中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2011年
5期
338-341
,共4页
孙雪梅%邵勇%王晓璐%吴味辛
孫雪梅%邵勇%王曉璐%吳味辛
손설매%소용%왕효로%오미신
妊娠并发症%胆汁淤积,肝内%滋养层%受体,胞质和核
妊娠併髮癥%膽汁淤積,肝內%滋養層%受體,胞質和覈
임신병발증%담즙어적,간내%자양층%수체,포질화핵
Pregnancy complications%Cholestasis,intrahepatic%Trophoblasts%Receptors,cytoplasmic and nuclear
目的 探讨组成型雄烷受体(CAR)在妊娠期肝内胆汁淤积症(ICP)孕妇胎盘合体滋养细胞中的表达及其临床意义.方法 收集2009年4月-2010年3月在重庆医科大学附属第一医院分娩的ICP孕妇的胎盘绒毛组织,其中ICP轻度孕妇10例(ICP轻度组),ICP重度孕妇10例(ICP重度组).同期健康晚期妊娠妇女10例为对照组.采用免疫组化链霉亲和素一生物素一过氧化物酶复合物(SABC)法检测胎盘组织中CAR蛋白的表达定位;采用逆转录(RT)-PCR技术检测CAR mRNA表达水平;蛋白印迹法检测CAR蛋白的表达水平.结果 (1)CAR表达定位在对照组和ICP轻度组胎盘合体滋养细胞中,着色较淡,呈淡棕黄色染色,着色部位主要在细胞质;在ICP重度组,CAR也主要定位在胎盘合体滋养细胞中,但着色部位主要在细胞核,呈棕黄色浓染.(2)对照组、ICP轻度组及重度组孕妇胎盘组织中CAR mRNA的表达水平分别为0.06±0.03、0.07 ±0.03及0.56 ±0.03,ICP重度组明显高于对照组和ICP轻度组,分别比较,差异均有统计学意义(P＜0.05);而ICP轻度组与对照组比较,差异无统计学意义(P＞0.05).(3)对照组、ICP轻度组及重度组孕妇胎盘组织中CAR蛋白的表达水平分别为0.74 ±0.03、0.79 ±0.03及1.02 ±0.04,ICP重度组与轻度组及对照组比较,差异均有统计学意义(P＜0.05);ICP轻度组与对照组比较,差异无统计学意义(P＞0.05).结论 ICP 孕妇尤其是ICP重度孕妇胎盘合体滋养细胞中CAR表达水平显著升高,其可能参与了维护胎盘的屏障功能,从而在ICP的发病中起到保护作用.
目的 探討組成型雄烷受體(CAR)在妊娠期肝內膽汁淤積癥(ICP)孕婦胎盤閤體滋養細胞中的錶達及其臨床意義.方法 收集2009年4月-2010年3月在重慶醫科大學附屬第一醫院分娩的ICP孕婦的胎盤絨毛組織,其中ICP輕度孕婦10例(ICP輕度組),ICP重度孕婦10例(ICP重度組).同期健康晚期妊娠婦女10例為對照組.採用免疫組化鏈黴親和素一生物素一過氧化物酶複閤物(SABC)法檢測胎盤組織中CAR蛋白的錶達定位;採用逆轉錄(RT)-PCR技術檢測CAR mRNA錶達水平;蛋白印跡法檢測CAR蛋白的錶達水平.結果 (1)CAR錶達定位在對照組和ICP輕度組胎盤閤體滋養細胞中,著色較淡,呈淡棕黃色染色,著色部位主要在細胞質;在ICP重度組,CAR也主要定位在胎盤閤體滋養細胞中,但著色部位主要在細胞覈,呈棕黃色濃染.(2)對照組、ICP輕度組及重度組孕婦胎盤組織中CAR mRNA的錶達水平分彆為0.06±0.03、0.07 ±0.03及0.56 ±0.03,ICP重度組明顯高于對照組和ICP輕度組,分彆比較,差異均有統計學意義(P＜0.05);而ICP輕度組與對照組比較,差異無統計學意義(P＞0.05).(3)對照組、ICP輕度組及重度組孕婦胎盤組織中CAR蛋白的錶達水平分彆為0.74 ±0.03、0.79 ±0.03及1.02 ±0.04,ICP重度組與輕度組及對照組比較,差異均有統計學意義(P＜0.05);ICP輕度組與對照組比較,差異無統計學意義(P＞0.05).結論 ICP 孕婦尤其是ICP重度孕婦胎盤閤體滋養細胞中CAR錶達水平顯著升高,其可能參與瞭維護胎盤的屏障功能,從而在ICP的髮病中起到保護作用.
목적 탐토조성형웅완수체(CAR)재임신기간내담즙어적증(ICP)잉부태반합체자양세포중적표체급기림상의의.방법 수집2009년4월-2010년3월재중경의과대학부속제일의원분면적ICP잉부적태반융모조직,기중ICP경도잉부10례(ICP경도조),ICP중도잉부10례(ICP중도조).동기건강만기임신부녀10례위대조조.채용면역조화련매친화소일생물소일과양화물매복합물(SABC)법검측태반조직중CAR단백적표체정위;채용역전록(RT)-PCR기술검측CAR mRNA표체수평;단백인적법검측CAR단백적표체수평.결과 (1)CAR표체정위재대조조화ICP경도조태반합체자양세포중,착색교담,정담종황색염색,착색부위주요재세포질;재ICP중도조,CAR야주요정위재태반합체자양세포중,단착색부위주요재세포핵,정종황색농염.(2)대조조、ICP경도조급중도조잉부태반조직중CAR mRNA적표체수평분별위0.06±0.03、0.07 ±0.03급0.56 ±0.03,ICP중도조명현고우대조조화ICP경도조,분별비교,차이균유통계학의의(P＜0.05);이ICP경도조여대조조비교,차이무통계학의의(P＞0.05).(3)대조조、ICP경도조급중도조잉부태반조직중CAR단백적표체수평분별위0.74 ±0.03、0.79 ±0.03급1.02 ±0.04,ICP중도조여경도조급대조조비교,차이균유통계학의의(P＜0.05);ICP경도조여대조조비교,차이무통계학의의(P＞0.05).결론 ICP 잉부우기시ICP중도잉부태반합체자양세포중CAR표체수평현저승고,기가능삼여료유호태반적병장공능,종이재ICP적발병중기도보호작용.
Objective To explore the expression and clinical significance of constitutive androstane receptor(CAR)in placenta syntrophoblast from patients with intrahepatic cholestasis of pregnancy(ICP).Methods Placenta were collected from women with ICP who delivered from April 2009 to March 2010 in First Affiliated Hospital of Chongqing Medical University.According to the severity of ICP,patients were classified into mild ICP group(n=10)and severe ICP group(n=10).Ten healthy pregnant women who delivered in the same period were chosen as control group.The location of CAR protein in placenta was studied by immunohistochemical streptavidin-biotin complex(SABC)method.CAR mRNA level was determined by reverse transcription(RT)-PCR technique and CAR protein expression level was determined by western blot.Results(1)CAR was located in the placenta syncytiotrophoblastic cells in control group and mild ICP group,showed light tan when stained,and was mainly in the cytoplasm.In severe ICP group,CAR was also located in placenta syncytiotrophoblastic cells but mainly in the nucleolus,showed dark tan when stained.(2)The mRNA expressions of CAR in control group,mild ICP group,severe ICP group were 0.06 ±0.03,0.07 ±0.03 and 0.56±0.03.respectively.CAR in severe ICP group was significantly higher than those in control group and mild ICP group(P＜0.05).The difference of mRNA between control group and mild ICP group wag not statistically significant(P＞0.05).(3)The CAR protein levels in control group,mild ICP group,severe ICP group were 0.74±0.03,0.79±01 03 and 1.02±0.04,respectively.CAR protein expression in the severe ICP group was significantly higher than the other two groups(P＜0.05).And there was no statistical significance between mild group and control group(P＞0.05).Conclusion In ICP women.especially severe ICP patients,the CAR expression in placenta syncytiotrophoblastic cells increased appreciably,which may be involved in the maintenance of placenta barrier function and protection in ICP pathogenesis.
