CAJ | 학술논문

目的探讨野生型耐药金黄色葡萄球菌的norA基因及其启动子的突变情况.方法分离并筛选出10株烧伤刨面的耐药性金黄色葡萄球菌,对norA基因及其启动子进行测序并分析其改变.结果共分离出金黄色葡萄球菌87株,它们对万古霉素100%敏感,对奎奴普汀和呋喃妥因也有很高的敏感性,而对其余抗生素的耐药率则非常高;耐甲氧西林金黄色葡萄球菌(MRSA)的阳性率达到91.7%.所有10株实验菌的norA基因编码区都有1 349位G→A的点突变,也就是氨基酸的291位处存在Gly(甘氨酸)→A8p(天冬氨酸)的突变.利血平逆转实验提示10株细菌对三种抗生素的MICs值均存在不同程度的下降.结论 norA基因突变是金黄色葡萄球菌耐药的机制之一.
목적 탐토야생형내약금황색포도구균적norA기인급기계동자적돌변정황.방법 분리병사선출10주소상포면적내약성금황색포도구균,대norA기인급기계동자진행측서병분석기개변.결과 공분리출금황색포도구균87주,타문대만고매소100%민감,대규노보정화부남타인야유흔고적민감성,이대기여항생소적내약솔칙비상고;내갑양서림금황색포도구균(MRSA)적양성솔체도91.7%.소유10주실험균적norA기인편마구도유1 349위G→A적점돌변,야취시안기산적291위처존재Gly(감안산)→A8p(천동안산)적돌변.리혈평역전실험제시10주세균대삼충항생소적MICs치균존재불동정도적하강.결론 norA기인돌변시금황색포도구균내약적궤제지일.
Objective To investigate the genetic mutation of the norA gene and its promotor from the wild-type drug-resistance Staphylococeus aureus(S.aureus)strains. Methods A total of 10 antibiotic-resistant S.aureus strains were isolated and screened from the burn wound for the sequencing and analysis of the nora gene and its promoter. Results There isolated 87 S.aureus strains from the burn wound flora,which were completely sensitive to vacomycin,highly sensitive to Quinupristin and Nitrofurantoin,but highly resistant to the other antibiotics,even up to91.7% of MRSA.There found the same point mutation(G→A) located at 1 349 sites of the norA gene coding region in all the S.aureus strains,saying that the amino acid was changed from Gly(glycin)to Asp(agpartic acid) in 291 sites.The resetpine reverse test showed that the MICs value of three antibiotics was lowered at various degrees in all 10strains.Conclusion NorA gene mutation is one of the mechanisms for antibiotic-resistance of S.aureus.