中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2011年
1期
21-24
,共4页
视网膜母细胞瘤/病理生理学%基质金属蛋白酶类%血管内皮生长因子类
視網膜母細胞瘤/病理生理學%基質金屬蛋白酶類%血管內皮生長因子類
시망막모세포류/병리생이학%기질금속단백매류%혈관내피생장인자류
Retinoblastoma/pathophysiology%Matrix metalloproteinases%Vascular endothelial growth factors
目的 观察基质金属蛋白酶(MMP)-2、MMP-9和血管内皮生长因子(VEGF)在视网膜母细胞瘤(RB)中的表达及其与RB分化程度和视神经浸润的关系.方法 经病理检查确诊的40例RB眼球石蜡标本纳入研究.按照RB细胞中有无菊形团排列以及RB肿瘤细胞是否浸润视神经分为分化型、未分化型以及视神经浸润、视神经无浸润组.其中,分化型15例,未分化型25例;视神经浸润13例,视神经无浸润27例.采用免疫组织化学方法 检测其MMP-2、MMP-9和VEGF的表达,对比分析MMP-2、MMP-9和VEGF表达与肿瘤病理组织分型和视神经是否侵润之间的关系.结果 40例RB中,MMP-2、MMP-9和VEGF的阳性表达率分别为52.5%、57.5%和72.5%.未分化型RB中MMP-2、MMP-9和VEGF的阳性表达均高于分化型RB中MMP-2、MMP-9和VEGF的阳性表达,差异有统计学意义(χ2=9.037,9.253,8.095;P＜0.05);有视神经浸润的RB中MMP-2、MM P-9和VEGF的阳性水平均高于无视神经浸润RB中MMP-2、MMP-9和VEGF的阳性水平,差异有统计学意义(χ2=11.045,10.243,8.956;P＜0.05).RB中MMP-2、MMP-9和VEGF的表达呈正相关关系(r=0.126,0.314;P＜0.05).结论 RB细胞内有MMP-2、MMP-9和VEGF的表达.MMP-2、MMP-9表达与VEGF表达存在相关性.MMP-2、MMP-9和VEGF的表达水平与视神经浸润存在密切的关系.
目的 觀察基質金屬蛋白酶(MMP)-2、MMP-9和血管內皮生長因子(VEGF)在視網膜母細胞瘤(RB)中的錶達及其與RB分化程度和視神經浸潤的關繫.方法 經病理檢查確診的40例RB眼毬石蠟標本納入研究.按照RB細胞中有無菊形糰排列以及RB腫瘤細胞是否浸潤視神經分為分化型、未分化型以及視神經浸潤、視神經無浸潤組.其中,分化型15例,未分化型25例;視神經浸潤13例,視神經無浸潤27例.採用免疫組織化學方法 檢測其MMP-2、MMP-9和VEGF的錶達,對比分析MMP-2、MMP-9和VEGF錶達與腫瘤病理組織分型和視神經是否侵潤之間的關繫.結果 40例RB中,MMP-2、MMP-9和VEGF的暘性錶達率分彆為52.5%、57.5%和72.5%.未分化型RB中MMP-2、MMP-9和VEGF的暘性錶達均高于分化型RB中MMP-2、MMP-9和VEGF的暘性錶達,差異有統計學意義(χ2=9.037,9.253,8.095;P＜0.05);有視神經浸潤的RB中MMP-2、MM P-9和VEGF的暘性水平均高于無視神經浸潤RB中MMP-2、MMP-9和VEGF的暘性水平,差異有統計學意義(χ2=11.045,10.243,8.956;P＜0.05).RB中MMP-2、MMP-9和VEGF的錶達呈正相關關繫(r=0.126,0.314;P＜0.05).結論 RB細胞內有MMP-2、MMP-9和VEGF的錶達.MMP-2、MMP-9錶達與VEGF錶達存在相關性.MMP-2、MMP-9和VEGF的錶達水平與視神經浸潤存在密切的關繫.
목적 관찰기질금속단백매(MMP)-2、MMP-9화혈관내피생장인자(VEGF)재시망막모세포류(RB)중적표체급기여RB분화정도화시신경침윤적관계.방법 경병리검사학진적40례RB안구석사표본납입연구.안조RB세포중유무국형단배렬이급RB종류세포시부침윤시신경분위분화형、미분화형이급시신경침윤、시신경무침윤조.기중,분화형15례,미분화형25례;시신경침윤13례,시신경무침윤27례.채용면역조직화학방법 검측기MMP-2、MMP-9화VEGF적표체,대비분석MMP-2、MMP-9화VEGF표체여종류병리조직분형화시신경시부침윤지간적관계.결과 40례RB중,MMP-2、MMP-9화VEGF적양성표체솔분별위52.5%、57.5%화72.5%.미분화형RB중MMP-2、MMP-9화VEGF적양성표체균고우분화형RB중MMP-2、MMP-9화VEGF적양성표체,차이유통계학의의(χ2=9.037,9.253,8.095;P＜0.05);유시신경침윤적RB중MMP-2、MM P-9화VEGF적양성수평균고우무시신경침윤RB중MMP-2、MMP-9화VEGF적양성수평,차이유통계학의의(χ2=11.045,10.243,8.956;P＜0.05).RB중MMP-2、MMP-9화VEGF적표체정정상관관계(r=0.126,0.314;P＜0.05).결론 RB세포내유MMP-2、MMP-9화VEGF적표체.MMP-2、MMP-9표체여VEGF표체존재상관성.MMP-2、MMP-9화VEGF적표체수평여시신경침윤존재밀절적관계.
Objective To observe the expression of matrix metalloproteinase(MMP-2, MMP-9 and vascular endothelial growth factor (VEGF) in retinoblastoma (RB) and its relationship with the differentiation and optic nerve infiltration of RB. Methods Forty paraffin specimens of pathological confirmed RB were studied. They were divided into differentiated group (15 cases) and undifferentiated group (25 cases) , optic nerve infiltration group( 13 cases) and without optic nerve infiltration group(27cases). The expression of MMP-2, MMP-9 and VEGF were detected by immunohistochemistry, their relationships with the differentiation and optic nerve infiltration were also analyzed. Results The positive rate of MMP-2, MMP-9 and VEGF expression in 40 RB cases were 52.5%, 57.5% and 72.5%respectively. The expression of MMP-2, MMP-9 and VEGF in the undifferentiated group were significantly higher than those in the differentiated group (χ2= 9. 037, 9. 253, 8. 095;P＜0. 05). The expression ofMMP-2, MMP-9 and VEGF in RB with optic nerve infiltration group were significantly higher than those in RB without optic nerve infiltration group (χ2=11.045,10. 243, 8. 956;P＜0. 05). The expression of MMP-2,MMP-9 had a positive correlation with the expression of VEGF in RB (r= 0. 126, 0. 314;P ＜ 0. 05).Conclusions MMP-2, MMP-9 and VEGF expressed in RB tumor tissues. The expression of MMP-2,MMP-9 has a positive correlation with the expression of VEGF. The levels of MMP-2, MMP-9 and VEGF expression are related to optic nerve infiltration of RB cells.
