CAJ | 학술논문

目的给予大鼠饲喂高脂饮食和灌注白酒分别制造大鼠高脂饮食性和酒精性脂肪肝模型，观察两种模型肝脏形态学和超微结构的区别。方法选用4～6周龄雄性SD大鼠45只，分为3组：正常对照组，只给予普通饲料；高脂饮食组，用高脂饲料喂养，连续8周；酒精组，用体积分数为60％白酒灌胃，初始剂量为8g/kg，每2周增加2 g，直到16g/kg维持16周。试验末，将动物麻醉，取部分肝组织制备病理切片，另取部分制备电镜。结果正常对照组大鼠肝组织形态结构正常，肝小叶规则，无脂滴发现；高脂饮食组可见多处脂质沉积，但尚无明显的肝纤维化表现，电镜下可见肝细胞胞浆内有大量脂肪滴积聚；酒精组脂肪肝大鼠肝细胞有脂肪变性的表现，电镜下可见类结晶体，线粒体肿胀，内质网扩张、断裂，数量较少，溶酶体内有电子密度增高的颗粒沉积，有一定量的脂滴。结论大鼠高脂饮食性脂肪肝超微结构改变主要以肝细胞细胞浆内脂肪滴沉积为主，而酒精性脂肪肝主要以线粒体肿胀和其他细胞器的变性和结构改变为主。
목적급여대서사위고지음식화관주백주분별제조대서고지음식성화주정성지방간모형，관찰량충모형간장형태학화초미결구적구별。방법선용4～6주령웅성SD대서45지，분위3조：정상대조조，지급여보통사료；고지음식조，용고지사료위양，련속8주；주정조，용체적분수위60％백주관위，초시제량위8g/kg，매2주증가2 g，직도16g/kg유지16주。시험말，장동물마취，취부분간조직제비병리절편，령취부분제비전경。결과정상대조조대서간조직형태결구정상，간소협규칙，무지적발현；고지음식조가견다처지질침적，단상무명현적간섬유화표현，전경하가견간세포포장내유대량지방적적취；주정조지방간대서간세포유지방변성적표현，전경하가견류결정체，선립체종창，내질망확장、단렬，수량교소，용매체내유전자밀도증고적과립침적，유일정량적지적。결론대서고지음식성지방간초미결구개변주요이간세포세포장내지방적침적위주，이주정성지방간주요이선립체종창화기타세포기적변성화결구개변위주。
Objective To evaluate morphologic and ultrasttuctural differences between fatty liver due to high fat diet and alcoholic fatty liver due to spirits. Methods Forty-five male SD rats aged 4～～6 weeks were divided into 3 groups: normal control group fed common feed; high fat diet group, continuously fed high fat diet for 8 weeks;alcoholic group: fed 60% spirits with initial dose of 8 g/kg, and doses were increased by 2 g/kg every 2 weeks until the dose reached 16 g/kg and then maintained for 16 weeks. At the end of the experiments, the animals were anesthetized and liver tiasues were obtained. One part of liver was used as pathologic sections and the other part was for electron microsoopy. Results In normal group, hepatic lobules were well-arranged and there were no lipid droplets; in high fat diet group, there were a lot of lipid deposition and generous fat accumulation was also obaerved by electron microscopy;in alcoholic fatty liver group, fatty degeneration of hepatocyte was observed. Similar crystal, swelling of mitochondrion, dilatation and disruption of endoplasmic reticulum as well as particles deposition of high electronic density and lipid droplet also were found by electron microscopy. Conclusion Main ultrastructural changes of fatty liver in rats fed high fat diet were lipid deposition in hepatocyte, though in alcoholic fatty liver, main changes were mitochondrion swclling and degeneration and structural alteration of other organellae.