清华大学学报(英文版)
清華大學學報(英文版)
청화대학학보(영문판)
TSINGHUA SCIENCE AND TECHNOLOGY
2003年
4期
502-507
,共6页
孙朝晖%赖燕来%左焕琮%叶智文%谢佐平
孫朝暉%賴燕來%左煥琮%葉智文%謝佐平
손조휘%뢰연래%좌환종%협지문%사좌평
mesencephalic progenitor cells%double grafts%Parkinson's disease%rotational behavior%fluorescent labeling
This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD).MPs were prepared from E11-13 rats and proliferated in the serum-free medium with basic fibroblast growth factor (bFGF) for 10 days.Cells were then collected and implanted into the striatum only-single grafts, or simultaneously into the substantia nigra (SN) and the striatum-double grafts.Twelve weeks after transplantation, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), a fluorescent dye, was microinjected into the ipsilateral striatum.The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs.Injection of the retrograde tracer DiI into the striatum results in fluorescent-labeled cells within the intranigral grafts only in double graft subjects.These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.