CAJ | 학술논문

目的研究我国苗族人群1型糖尿病(T1DM)患者与HLA-DR基因频率的相关性.方法选择无血缘关系的纯苗族T1DM患者45例,纯汉族T1DM患者43例,纯苗族健康对照(NC)组52例,纯汉族NC组58例.运用序列特异性引物-聚合酶链反应(PCR/SSP)技术测定HLA-DR1-10基因频率.结果(1)苗族及汉族T1DM组患者的DR2基因频率分别明显低于苗族及汉族NC组(P＜0.05);DR3、DR9基因频率分别高于NC组(P＜0.05).(2)苗族T1DM组及苗族NC组的DR5基因频率分别明显高于汉族T1DM组及汉族NC组(P＜0.05).(3)苗族T1DM组的DR2基因频率明显低于汉族NC组(P＜0.05);DR3、DR5、DR9基因频率分别高于汉族NC组(P＜0.05).(4)苗族及汉族人群中,T1DM组的DR2基因频率分别明显低于NC组,其相对危险度RR＜1,P＜0.05;DR3、DR9基因频率分别高于NC组,其相对危险度RR＞1,P＜0.05.结论苗族T1DM组与汉族T1DM组患者HLA-DR1、DR2、DRa、DR3、DR6、DR7、DR8、DR9、DR10基因频率差异无统计学意义,苗族T1DM组及苗族NC组DR5基因频率高于汉族T1DM组及汉族NC组.DR3、DR9与T1DM呈正相关;DR2与T1DM呈负相关.
목적연구아국묘족인군1형당뇨병(T1DM)환자여HLA-DR기인빈솔적상관성.방법선택무혈연관계적순묘족T1DM환자45례,순한족T1DM환자43례,순묘족건강대조(NC)조52례,순한족NC조58례.운용서렬특이성인물-취합매련반응(PCR/SSP)기술측정HLA-DR1-10기인빈솔.결과(1)묘족급한족T1DM조환자적DR2기인빈솔분별명현저우묘족급한족NC조(P＜0.05);DR3、DR9기인빈솔분별고우NC조(P＜0.05).(2)묘족T1DM조급묘족NC조적DR5기인빈솔분별명현고우한족T1DM조급한족NC조(P＜0.05).(3)묘족T1DM조적DR2기인빈솔명현저우한족NC조(P＜0.05);DR3、DR5、DR9기인빈솔분별고우한족NC조(P＜0.05).(4)묘족급한족인군중,T1DM조적DR2기인빈솔분별명현저우NC조,기상대위험도RR＜1,P＜0.05;DR3、DR9기인빈솔분별고우NC조,기상대위험도RR＞1,P＜0.05.결론묘족T1DM조여한족T1DM조환자HLA-DR1、DR2、DRa、DR3、DR6、DR7、DR8、DR9、DR10기인빈솔차이무통계학의의,묘족T1DM조급묘족NC조DR5기인빈솔고우한족T1DM조급한족NC조.DR3、DR9여T1DM정정상관;DR2여T1DM정부상관.
Objective To study the correlation between type 1 diabetes（T1DM） in Chinese Miao population and HLA-DR genetic frequency. Methods Forty five T1DM in unadulterated Miao population and 43 T1DM in unadulterated Han population were studied. The patients between the two groups had no kinship. Fifty two healthy unadulterated Miao population and 58 healthy unadulterated Han population were controls. The sequence-specific primer-polymerase chain reaction （PCR-SSP） techniques were adopted to test the frequencies of HLA-DR1-10. Results （1） DR2 frequency of T1DM in Miao and Han population was lower than that of the healthy Miao and Han population （P<0.05）. Their DR3, DR9 frequencies were higher than those of healthy Miao and Han population respectively（all P<0.05）. （2） DR5 frequencies of T1DM and healthy Miao population were higher than those of T1DM and healthy Han population（P<0.05）. （3） DR2 genetic frequency of T1DM Miao population was lower than that of the healthy Han population （P<0.05）. DR3,DR5,DR9 frequencies were respectively higher in T1DM Miao population than those of healthy Han population （P<0.05）. （4） DR2 frequencies of T1DM Miao and Han population were lower than those of the healthy Miao and Han population with relative risk<1, P<0.05. Their DR3, DR9 frequencies were higher than those of healthy Miao and Han population respectively with relative risk >1, P<0.05. Conclusions There is no obvious difference in HLA-DR1,DR2,DR3,DR4,DR6,DR7,DR8,DR9,DR10 frequencies between T1DM Miao and Han population. DR5 frequencies of T1DM and healthy Miao population are higher than those of T1DM and healthy Han population. The DR3 and DR9 are in positive correlation with T1DM, the DR2 is in negative correlation with T1DM.