CAJ | 학술논문

建立了高效液相色谱法-荧光检测器测定树叶中的黄酮(槲皮素,桑色素和山奈酚)含量的方法.采用Polaris C18色谱柱(2.0m×100mm,3μm),柱温60℃;流动相,A相为0.1mol/L HAc,B相为乙腈,流速0.3mL/min;梯度淋洗,柱后衍生;激发波长438nm,发射波长483nm.槲皮素、桑色素和山奈酚的线性范围及相关系数分别为,1.720~13.76mg/L,r=1.000;0.222~0.888mg/L,r=0.9997; 0.965~7.720mg/L,r=0.9991.平均回收率在90.0%~101.5%之间.该方法简便、准确,可用于植物中槲皮素,桑色素和山奈酚含量的测定.
건립료고효액상색보법-형광검측기측정수협중적황동(곡피소,상색소화산내분)함량적방법.채용Polaris C18색보주(2.0m×100mm,3μm),주온60℃;류동상,A상위0.1mol/L HAc,B상위을정,류속0.3mL/min;제도림세,주후연생;격발파장438nm,발사파장483nm.곡피소、상색소화산내분적선성범위급상관계수분별위,1.720~13.76mg/L,r=1.000;0.222~0.888mg/L,r=0.9997; 0.965~7.720mg/L,r=0.9991.평균회수솔재90.0%~101.5%지간.해방법간편、준학,가용우식물중곡피소,상색소화산내분함량적측정.
A method of high performance liquid chromatography (HPLC) with fluorescence detector was established for quantitative determination of flavonoids (quercetin, morin hydrate and kaempferol) in tree leaves. The flavonoids were separated on a Polaris C18 column (2.0m×100mm,3μm) at 60℃. Mobile phase A was 0.1 mol/L HAc and mobile phase B was acetonitrile, with the flow rate of 0.3 mL/min. The gradient elution and post-column derivatization were employed. The excitation wavelength and emission wavelength were 438nm and 483nm, respectively. The linear ranges were 1.720~13.76mg/L (r=1.0000) for quercetin, 0.222~0.888mg/L (r=9997) for morin hydrate, and 0.965~7.720mg/L (r=0.9991) for kaempferol. The average recoveries were 90.0%~101.5%. The method is simple and accurate, and can be used for the determination of quercetin, morin hydrate and kaempferol in plants.