医学分子生物学杂志
醫學分子生物學雜誌
의학분자생물학잡지
FOREIGN MEDICAL SCIENCES
2009年
4期
325-330
,共6页
王志富%周钢桥%方宇%张红星%智联腾%张秀梅%翟芸%乐伟%冯凯%刘保池%陈惠鹏%凌焱%李玉霞%贺褔初
王誌富%週鋼橋%方宇%張紅星%智聯騰%張秀梅%翟蕓%樂偉%馮凱%劉保池%陳惠鵬%凌焱%李玉霞%賀褔初
왕지부%주강교%방우%장홍성%지련등%장수매%적예%악위%풍개%류보지%진혜붕%릉염%리옥하%하부초
脓毒症%单核苷酸多态性%Toll样受体5%遗传关联
膿毒癥%單覈苷痠多態性%Toll樣受體5%遺傳關聯
농독증%단핵감산다태성%Toll양수체5%유전관련
sepsis%single nucleotide polymorphism%TLR5%genetic association
目的 探讨Toll样受体5(Toll-like receptor 5, TLR5)基因多态性位点与脓毒症发生风险及疾病严重程度的相关性.方法 采用病例-对照研究设计,募集了255例脓毒症患者和260例对照个体.应用贝克曼公司的商用SNPstream 分型技术和PCR-RFLP 方法对TLR5基因的3个编码区多态性位点进行分型.采用Logistic 回归分析,校正性别、年龄、吸烟和饮酒、慢性病状态、APACHEⅡ评分和脓毒症病因等混杂因素的影响,评价多态性位点与脓毒症的发生风险,以及脓毒症性休克、死亡和器官功能障碍等表型的遗传相关性.结果 TLR5基因的3个多态性位点在病例和对照组中的基因型分布均呈哈-温平衡状态.这3个编码区的多态性位点与脓毒症的发生风险和疾病严重程度均无遗传学关联.结论 TLR5基因的多态性位点可能在脓毒症的发生、发展和病程转归中不发挥重要作用.
目的 探討Toll樣受體5(Toll-like receptor 5, TLR5)基因多態性位點與膿毒癥髮生風險及疾病嚴重程度的相關性.方法 採用病例-對照研究設計,募集瞭255例膿毒癥患者和260例對照箇體.應用貝剋曼公司的商用SNPstream 分型技術和PCR-RFLP 方法對TLR5基因的3箇編碼區多態性位點進行分型.採用Logistic 迴歸分析,校正性彆、年齡、吸煙和飲酒、慢性病狀態、APACHEⅡ評分和膿毒癥病因等混雜因素的影響,評價多態性位點與膿毒癥的髮生風險,以及膿毒癥性休剋、死亡和器官功能障礙等錶型的遺傳相關性.結果 TLR5基因的3箇多態性位點在病例和對照組中的基因型分佈均呈哈-溫平衡狀態.這3箇編碼區的多態性位點與膿毒癥的髮生風險和疾病嚴重程度均無遺傳學關聯.結論 TLR5基因的多態性位點可能在膿毒癥的髮生、髮展和病程轉歸中不髮揮重要作用.
목적 탐토Toll양수체5(Toll-like receptor 5, TLR5)기인다태성위점여농독증발생풍험급질병엄중정도적상관성.방법 채용병례-대조연구설계,모집료255례농독증환자화260례대조개체.응용패극만공사적상용SNPstream 분형기술화PCR-RFLP 방법대TLR5기인적3개편마구다태성위점진행분형.채용Logistic 회귀분석,교정성별、년령、흡연화음주、만성병상태、APACHEⅡ평분화농독증병인등혼잡인소적영향,평개다태성위점여농독증적발생풍험,이급농독증성휴극、사망화기관공능장애등표형적유전상관성.결과 TLR5기인적3개다태성위점재병례화대조조중적기인형분포균정합-온평형상태.저3개편마구적다태성위점여농독증적발생풍험화질병엄중정도균무유전학관련.결론 TLR5기인적다태성위점가능재농독증적발생、발전화병정전귀중불발휘중요작용.
Objective Toll-like receptor 5 (TLR5) is able to recognize bacterial flagellin of both Gram-negative and -positive bacteria, activate NF-κB signal pathway and play a central role in sepsis. In the present study, we investigated whether the polymorphisms of TLR5 gene is associated with susceptibility to and disease severity of sepsis.Methods Three TLR5 polymorphisms, R392X (rs5744168), S592N (rs2072493) and L616F (rs5744174) in exon 6, were genotyped in 255 patients with sepsis and 260 control subjects by the Beckman SNPstream genotyping platform and PCR-RFLP analysis. The associations with susceptibility to and disease severity of sepsis were estimated by logistic regression, with controlling for confounding factors, including age, sex, smoking status, drinking status, chronic diseases status, APACHEII and critical illness status.Result Genotype frequencies of these three polymorphisms were conformed to the Hardy-Weinberg equilibrium in both patients and controls. On the basis of logistic regression analysis with adjustment for confounding factors, no significant association was found between polymorphisms of and susceptibility to sepsis. Also no significant association with septic shock, sepsis caused death and the number of organ dysfunction was found within the three polymorphisms.Conclusion Our findings suggest that the TLR5 polymorphisms R392X, S592N and L616F do not play a major role in mediating susceptibility to and disease severity of sepsis.
