CAJ | 학술논문

利用气体循环培养体系从陕西乾县HUP-豆科植物紫花苜蓿(Medicago sativa)根际土壤中分离获得37株细菌.菌株氧化氢能力测定结果表明,8株菌氧化氢和自养生长能力较强,初步确定为氢氧化细菌类群;根据其形态特征、培养特征和生理生化特性,鉴定为7个不同属:假单胞菌属(Pseudomonas)、邻单胞菌属(Plesiomonas)、脂肪杆菌属(Pimelobacter)、黄色杆菌属(Xanthobacter)、勒米诺氏菌属(Leminorella)、地杆菌属(Terrabacter)和稀有杆菌属(Rarobacter);其中氧化氢能力最强的优势菌株WMQ-7 16S rDNA序列(GenBank登录号为EU807744)长度为1 451bp,GC含量为53.8%,其核苷酸序列与假单胞菌属同源性高于99%,在系统发育树上位于同一分支,将WMQ-7菌株鉴定为假单胞菌属(Pseudomonas).图2表5参16
이용기체순배배양체계종협서건현HUP-두과식물자화목숙(Medicago sativa)근제토양중분리획득37주세균.균주양화경능력측정결과표명,8주균양화경화자양생장능력교강,초보학정위경양화세균류군;근거기형태특정、배양특정화생리생화특성,감정위7개불동속:가단포균속(Pseudomonas)、린단포균속(Plesiomonas)、지방간균속(Pimelobacter)、황색간균속(Xanthobacter)、륵미낙씨균속(Leminorella)、지간균속(Terrabacter)화희유간균속(Rarobacter);기중양화경능력최강적우세균주WMQ-7 16S rDNA서렬(GenBank등록호위EU807744)장도위1 451bp,GC함량위53.8%,기핵감산서렬여가단포균속동원성고우99%,재계통발육수상위우동일분지,장WMQ-7균주감정위가단포균속(Pseudomonas).도2표5삼16
Gas-cycle incubation system was used to enrich the Medicago sativa rhizosphere soil collected in Shaanxi Province, China and 37 strains of bacteria were isolated. Among them, 8 stains had stronger ability to oxidize hydrogen and could grow chemolithoautotrophically, so they were preliminarily identified as hydrogen-oxidizing bacteria. According to their cells, colony forms and biochemical characteristics, the 8 strains were identified as Pseudomonas, Plesiomonas, Pimelobacter, Xanthobacter, Leminorella, Terrabacter and Rarobacte. Strain WMQ-7 had the strongest ability to oxidize hydrogen, and its 16S rDNA sequence (GenBank accession number EU807744) was 1 451 bp and GC content was 53.8%. Strain WMQ-7 was clustered together with Pseuclomonas in phylogenic tree, with the sequence identity of 99%, Therefore, strain WMQ-7 was identified as Pseudomonas. Fig 2, Tab 5, Ref 16