CAJ | 학술논문

目的:制备载卡莫司汀脂质超声造影剂,检测其物理性状与造影增强效果.方法:采用机械振荡法制备载卡莫司汀脂质超声造影剂,显微镜下观察并计算其浓度、马尔文测量粒径大小、分布、电位,高效液相色谱法测量药物的包封率.微泡经冷冻干燥与~(60)Co辐照消毒后,经兔耳缘静脉注射,观察其在兔肝内显像效果.结果:载药脂质造影剂的浓度为(2.6～3.1)×10~9/ml,平均粒径为(1.05±0.20)μm;卡莫司汀包封率为42.6%±0.11%;电位为-(12.93±1.84)mV;经兔耳缘静脉注射后,兔肝内可见持续的增强显像效果.结论:成功制备的卡莫司汀脂质超声造影剂,有望为肿瘤的靶向治疗提供新方向.
목적:제비재잡막사정지질초성조영제,검측기물이성상여조영증강효과.방법:채용궤계진탕법제비재잡막사정지질초성조영제,현미경하관찰병계산기농도、마이문측량립경대소、분포、전위,고효액상색보법측량약물적포봉솔.미포경냉동간조여~(60)Co복조소독후,경토이연정맥주사,관찰기재토간내현상효과.결과:재약지질조영제적농도위(2.6～3.1)×10~9/ml,평균립경위(1.05±0.20)μm;잡막사정포봉솔위42.6%±0.11%;전위위-(12.93±1.84)mV;경토이연정맥주사후,토간내가견지속적증강현상효과.결론:성공제비적잡막사정지질초성조영제,유망위종류적파향치료제공신방향.
Objective: To preparate BCUN-loaded lipid ultrasound contrast agent and detect its physical properties and contrast-enhancing effects. Methods: Mechanical oscillation was used to prepare BCUN-loaded lipid contrast agent. Density was detected and analyzed with microscope. Malvem determined intensity,disposition and Zeta potential. RP-HPLC detected envelopment rate. Cryodesiccated-microb ubbles were sterilized by ~(60)Co irradiation. Following injected it through rabbit ear vein, effects of ultrasounic enhanced imaging in rabbit liver was observed. Results: The density of microbubble was (2.6-3.1) × 10~7 ml. The mean intensity was (1.05 ± 0.20) μm. The mean Zeta potential was (12.93 ± 1.84) mV. The mean nvelopment rate was (42.6 ± 0.11)%. After injecting this microbubble, good and sustained ultrasounic enhanced images in rabbit liver were seen.Conclusions: BCUN- loaded lipid contrast agents were successfully prepared,which hopefully provide a new option of targeting treatment for the tumor.